Volume : 08, Issue : 12, December – 2021
Title:
51.ESTIMATION OF ELLAGIC ACID FROM FRAGARIA VESCA BY CHROMATOGRAPHY
Authors :
Mr.MD. Yakub Pasha*, Dr.T. Mangilal
Abstract :
Ellagic acid, a dimeric comound of Gallic acid is found in various fruits and vegetables. They are available in the form of hydrolysable tannins called as ellagitannins. The aim of the present study is to develop and validate a simple, fast, specific and precise HPTLC method for the estimation of ellagic acid in fresh and processed fruit products. Aluminium plates precoated with silica gel 60 GF254 was used as stationary phase and a mixture of toulene: ethyl acetate: formic acid in the ratio of 5:5:2.5 (v/v) allagic acids mobile phase. Quantitation was carried out by scanning the developed spots using a densitometer in absorbance mode at 254nm. The Rf value of Ellagic acid was 0.35. The result of analysis has been validated stastically and by recovery studies. Linearity was observed in the concentration range of 400-1200 ng/spot. HPTLC method for the estimation of ellagic acid was developed and validated. Thus it may be used for the estimation of ellagic acid in fresh and processed fruits.
Key Words: Ellagic Acid,HPTLC,Fresh Fruits,Processed Fruit Products.
Cite This Article:
Please cite this article in press Md.Yakub Pasha et al, Estimation Of Ellagic Acid From Fragaria Vesca By Chromatography, Indo Am. J. P. Sci, 2021; 08(12).
Number of Downloads : 10
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