Volume : 11, Issue : 12, December – 2024
Title:
RP-HPLC METHOD AND ITS VALIDATION FOR ANALYSIS OF FLUOXETINE HCL AND ALPRAZOLAM IN BULK AND PHARMACEUTICAL DOSAGE FORM
Authors :
Syeda Rabab Fatima*, Dr. G.Vijaya Kumar
Abstract :
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Fluoxetine HCL and Alprazolam, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Zorbax C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: Phosphate Buffer pH 3.9 (55:45v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 255nm. The retention time of the Fluoxetine HCL and Alprazolam was 2.061, 2.462 ±0.02min respectively. The method produces linear responses in the concentration range of 1-5µg/ml of Fluoxetine HCL and 100-500µg/ml of Alprazolam. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Keywords: Fluoxetine HCL, Alprazolam, RP-HPLC, validation.
Cite This Article:
Please cite this article in press Syeda Rabab Fatima et al Rp-Hplc Method And Its Validation For Analysis Of Fluoxetine Hcl And Alprazolam In Bulk And Pharmaceutical Dosage Form.,Indo Am. J. P. Sci, 2024; 11 (12).
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