Volume : 12, Issue : 04, April – 2025

Title:

OPTIMIZATION OF ANALYTICAL PARAMETERS FOR ROPINIROLE DETERMINATION – A REVIEW

Authors :

Banothu Bhadru, Puppala Madhuri, Motapalukula Shivani, Patil Manmath, Tadikonda Rama Rao

Abstract :

Ropinirole, a selective non-ergot dopamine D2/D3 receptor agonist, is widely used for the treatment of Parkinson’s disease (PD) and Restless Legs Syndrome (RLS). Its therapeutic relevance necessitates accurate, sensitive, and reliable analytical methods for its determination in pharmaceutical formulations. This review highlights the optimization of various analytical parameters for ropinirole determination using UV spectroscopy, High-Performance Liquid Chromatography (HPLC), High-Performance Thin Layer Chromatography (HPTLC), and Ultra-High-Performance Liquid Chromatography (UHPLC). The methods differ in mobile phase composition, solvents, detection wavelengths, linearity range, Limit of Detection (LOD), and Limit of Quantization (LOQ). Comparative data from multiple references illustrate the suitability and sensitivity of each method. HPLC and UHPLC techniques demonstrated higher sensitivity and broader linearity ranges, making them more favorable for routine analysis. This review serves as a valuable resource for researchers and analysts aiming to select or optimize the most effective analytical approach for ropinirole quantification in various pharmaceutical and research applications.
Keywords: Ropinirole, Parkinson’s, UV, HPLC, HPTLC, UHPLC

Cite This Article:

Please cite this article in press Banothu Bhadru et al., Optimization Of Analytical Parameters For Ropinirole Determination – A Review.,, Indo Am. J. P. Sci, 2025; 12(04).

Number of Downloads : 10

References:

1. Sudha M, Sharma NK, Islam S, Bhuyan SH, Illendula S, Suresh CH, Rao KN. Method development and validation of ropinirole by UV spectroscopy method. World Journal of Pharmacy and Pharmaceutical Sciences. 2022; 11(8):1418-1427
2. Kothapalli LP, Choudhari ME, Thomas AB, Nanda RK, Deshpande AD, Gaidhani PS. Validated RP- HPLC and Spectrophotometric determination of Ropinirole hydrochloride in bulk and in pharmaceutical dosage form. Der Pharma Chemica. 2011; 3(6):1-9.
3. Sreekanth N, Rao B, Mukkanti K. RP-HPLC method development and validation of ropinirole hydrochloride in bulk and pharmaceutical dosage forms. Int J Pharm Sci. 2009; 1(1):186-92.
4. Azeem A, Iqbal Z, Ahmad FA, Khar RK, Talegaonkar S. Development and validation of a stability-indicating method for determination of ropinirole in the bulk drug and in pharmaceutical dosage forms. Acta chromatographica. 2008; 20(1):95-107.
5. Sadashivaiah R, Rohith G, Satheesha Babu BK. Quantification of ropinirole hydrochloride in api and tablets by novel stability-indicating RP-HPLC method: It’s validation and forced degradation studies. Int. J. Appl. Pharm. 2019; 11:317-23.
6. Kakouris A, Samara V, Kalaskani A, Panderi I. Simultaneous determination of impurities in ropinirole tablets by an improved HPLC method coupled with diode array detection. Chromatographia. 2014; 77:447-57.
7. Hefnawy M, Al-Majed A, Mohammed M, Attia S, Rizk M, El-Eryan RT. Development of a monolithic HPLC-ECD method for the determination of ropinirole HCl in mouse sera and dosage forms. Br J Pharm Res. 2016; 13:1-1.
8. Anilkumar A. method development and validation for the estimation of ropinirole HCL in tablets by RP-HPLC. International Journal of Advances in Pharmaceutical Analysis.2012; 2 (3): 68-72
9. Gummadi S, Siyyadri DS. Development and validation of RP-HPLC method for the determination of ropinirole hydrochloride in bulk and pharmaceutical dosage forms. Research Journal of Pharmacy and Technology. 2022; 15(4):1761-7.
10. Önal A. Method Development and Validation of a Rapid Determination of Ropinirole in Tablets by LC-UV. Chromatographia. 2006; 64: 459-461
11. Fuster J, Negro S, Salama A, Fernández-Carballido A, Marcianes P, Boeva L, Barcia E. HPLC-UV method development and validation for the quantification of ropinirole in new PLGA multiparticulate systems: Microspheres and nano particles. International journal of pharmaceutics. 2015; 491(2):310-7.
12. Parmar G, Sharma S, Singh K, Bansal G. Forced degradation study to develop and validate stability-indicating RP-HPLC for quantification of ropinirole hydrochloride in its modified release tablets. Chromatographia. 2009; 69:199-206.
13. Mustafa G, Baboota S, Ali J, Ahuja A. Design expert-supported development and validation of high-performance thin-layer chromatographic stability-indicating (HPTLC) method: an application in quantitative analysis of ropinirole in the bulk drug and in marketed dosage forms. Journal of Pharmaceutical Innovation. 2012; 7:47-55.
14. Mustafa G, Ahuja A, Baboota S, Ali J. Box-Behnken supported validation of stability-indicating high performance thin-layer chromatography (HPTLC) method: An application in degradation kinetic profiling of ropinirole. Saudi Pharmaceutical Journal. 2013; 21(1):93-102.
15. Bari SB, Bakhshi AR, Jain PS, Surana SJ. Development and Validation of Stability-Indicating TLC-Densitometric Determination of Ropinirole Hydrochloride in Bulk and Pharmaceutical Dosage Form. Pharm Anal 2011; 2(4): 2-7.
16. Mustafa G, Ahmad N, Baboota S, Ali J, Ahuja A. Stressed Kinetics of Nano emulsion Formulation Encapsulated Ropinirole with a Validated Ultra High Performance Liquid Chromatography–Synapt Mass Spectrometry (UPLC‐MS/MS ESI‐Q‐TOF). Journal of the Chinese Chemical Society. 2012; 59(8):1021-30.
17. Mustafa G, Ahmad N, Baboota S, Ali J, Ahuja A. UHPLC/ESI‐Q‐TOF‐MS method for the measurement of dopamine in rodent striatal tissue: A comparative effects of intranasal administration of ropinirole solution over nano emulsion. Drug Testing and Analysis. 2013; 5(8):702-9.
18. Tome T, Obreza A, Casar Z. Developing an improved UHPLC method for efficient determination of European Pharmacopeia process-related impurities in ropinirole hydrochloride using analytical quality by design principles. Molecules. 2020; 25(11):2691.