Volume : 12, Issue : 03, March – 2025
Title:
IN VITRO ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF AQUEOUS EXTRACT OF HOLARRHENA ANTIDYSENTERICA
Authors :
Palak Sahu, Priya Prasad, Prachi Yaduwanshi, Dr. Bhawna Sharma*, Dr. Jaswinder Mehta, Dr. Ruchi Acharya, Dr. Peenu Mahendra Joshi
Abstract :
The present study investigates the in vitro antioxidant and antimicrobial properties of the aqueous extract of Holarrhena antidysenterica, a medicinal plant widely recognized in traditional systems for treating gastrointestinal and infectious diseases. The extract was subjected to preliminary phytochemical screening, which confirmed the presence of glycosides, saponins, phenols, tannins, proteins, sterols, and carbohydrates. The antioxidant potential was evaluated using the DPPH radical scavenging method, and the extract exhibited dose-dependent activity with a maximum inhibition of 42.26% at 100 µg/ml and an IC₅₀ value of 88.84 µg/ml, compared to ascorbic acid (IC₅₀: 20.67 µg/ml). Antimicrobial activity was assessed against Staphylococcus aureus and Klebsiella pneumoniae using the agar well diffusion method. The extract showed moderate antibacterial activity, with a maximum zone of inhibition of 11.3 mm and 10.3 mm, respectively, at the highest tested concentration (100 mg/ml), while ciprofloxacin exhibited significantly larger zones. These findings support the traditional therapeutic use of Holarrhena antidysenterica and indicate its potential as a source of natural antioxidant and antimicrobial agents.
Keywords: Holarrhena antidysenterica, antioxidant activity, DPPH assay, antimicrobial activity, aqueous extract, phytochemical screening, Staphylococcus aureus, Klebsiella pneumoniae
Cite This Article:
Please cite this article in press Palak Sahu et al., In Vitro Antioxidant And Antimicrobial Activities Of Aqueous Extract Of Holarrhena Antidysenterica., Indo Am. J. P. Sci, 2025; 12(05).
Number of Downloads : 10
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