Volume : 12, Issue : 08, August- 2025
Title:
REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF SPIRONOLACTONE AND HYDROCHLOROTHIAZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM
Authors :
A.Jhansi, M. Chaitanya
Abstract :
The present research work describes a novel, simple, accurate, sensitive, rapid reversed-phase liquid chromatographic method for simultaneous estimation of Spironolactone & Hydrochlorothiazide in bulk & pharmaceutical formulations. The chromatographic separation was achieved on WATERS HPLC with PDA detector and column Symmetry, phosphate buffer and methanol as mobile phase at a flow rate of 1.0ml/min. The detection was carried out at 250 nm. The retention time of Spironolactone & Hydrochlorothiazide was found to be 3.388and 1.688. %Recoveries obtained for Spironolactone and Hydrochlorothiazide were 99.87% & 100.3%.The %RSD below 2.0 shows the high precision of proposed method. The method was validated for precision, Recovery, Specify Detection & Quantification limits in accordance with ICH guidelines.
Keywords: Spironolactone, Hydrochlorothiazide RP-HPLC, Validation.
Cite This Article:
Please cite this article in press M. Chaitanya et al., Reverse Phase High Performance Liquid Chromatography Method For Simultaneous Estimation Of Spironolactone And Hydrochlorothiazide In Bulk And Pharmaceutical Dosage Form., Indo Am. J. P. Sci, 2025; 12(08).
Number of Downloads : 10
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