Volume : 12, Issue : 11, November – 2025
Title:
PHYTOCHEMICAL INVESTIGATION AND WOUND HEALING ACTIVITY OF Aphanamixis polystachya BARK EXTRACT
Authors :
Vishnu Kant Gupta*, Dr. Ashish Chourasia, Payal Saiju, Dr. Nishi Prakash Jain
Abstract :
The present study aimed to investigate the phytochemical constituents, antioxidant potential, and wound healing activity of Aphanamixis polystachya bark extract. Successive extraction was carried out using ethyl acetate, ethanol, and water as solvents, and the extractive values indicated that the aqueous extract yielded the highest percentage (8.21% w/w), followed by the ethanolic (6.70% w/w) and ethyl acetate extract (1.05% w/w). Preliminary phytochemical screening revealed the presence of alkaloids, glycosides, flavonoids, tannins, phenols, diterpenes, and proteins predominantly in the ethanolic extract. Quantitative estimation showed that the ethanolic extract contained a higher total phenolic (0.783 mg/100 mg) and flavonoid content (0.925 mg/100 mg) compared to the other extracts. The antioxidant activity assessed by the DPPH method demonstrated a concentration-dependent increase in radical scavenging with an IC₅₀ value of 92.81 µg/mL for the ethanolic extract, indicating moderate antioxidant potential. The wound healing activity was evaluated using an excision wound model in rats, where the 2% Aphanamixis polystachya extract gel showed significant wound contraction (85.9% by day 11) compared to the control and was comparable to the standard Betadine group (92.3%). These findings suggest that the ethanolic extract of Aphanamixis polystachya possesses notable antioxidant and wound healing activities, likely attributed to its rich content of phenolic and flavonoid compounds, which play a vital role in tissue repair and protection against oxidative stress.
Keywords: Aphanamixis polystachya, phytochemical screening, antioxidant activity, DPPH assay, total phenolic content, wound healing; ethanolic extract.
Cite This Article:
Please cite this article in press Vishnu Kant Gupta et al., Phytochemical Investigation And Wound Healing Activity Of Aphanamixis Polystachya Bark Extract, Indo Am. J. P. Sci, 2025; 12(11).
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