Volume : 13, Issue : 02, February – 2026
Title:
DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF METRONIDAZOLE AND NALIDIXIC ACID BY RP-HPLC
Authors :
Kommuri Hari Krishna Reddy*, Godasu Suresh kumar, Dr. P. Viahnu priya.
Abstract :
A simple, rapid, precise, and reproducible Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the simultaneous estimation of Metronidazole and Nalidixic Acid in bulk and pharmaceutical dosage forms. Chromatographic separation was carried out using a Phenomenex Luna C18 column (4.6 × 150 mm, 5 µm) with a mobile phase consisting of Methanol and Water (70:30 v/v), delivered at a flow rate of 1 mL/min. The column temperature was maintained at 35 °C, the detection wavelength was set at 230 nm, and the injection volume was 10 µL with a total run time of 10 minutes.
Both drugs were well resolved with sharp, symmetrical peaks, and the method was validated as per ICH guidelines. The results demonstrated excellent linearity, accuracy, precision, specificity, and robustness, with recovery studies confirming accuracy and %RSD values within acceptable limits (<2%). The developed method proved to be sensitive and free from interference of excipients.
Keywords: RP-HPLC, Metronidazole and Nalidixic Acid, simultaneous estimation, validation, Phenomenex Luna C18 column, ICH guidelines.
Cite This Article:
Please cite this article in press Kommuri Hari Krishna Reddy et al., Development And Validation Of Analytical Method For Simultaneous Estimation Of Metronidazole And Nalidixic Acid By Rp-Hplc, Indo Am. J. P. Sci, 2026; 13(02).
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