Volume : 13, Issue : 02, February – 2026
Title:
A REVIEW ON THE WOUND-HEALING POTENTIAL AND ANALYTICAL STANDARDIZATION OF TECOMA STANS ETHANOLIC GEL
Authors :
Mohammad Tausif Mohammad Anis, Prof. Anjali Rathod
Abstract :
Wound healing is a complex biological process that becomes significantly impaired in diabetic conditions due to oxidative stress, poor circulation, and prolonged inflammation. Tecoma stans is a medicinal plant known for its antioxidant, anti-inflammatory, antimicrobial, and antidiabetic properties. This review summarizes the phytochemical composition, pharmacological activities, gel formulation approach, and analytical standardization of Tecoma stans for wound management. The presence of flavonoids, alkaloids, phenolic compounds, and naphthoquinones contributes to its therapeutic effectiveness. Incorporation of the ethanolic extract into a topical gel system enhances stability, controlled release, and patient compliance. Analytical techniques such as UV–Visible spectroscopy and HPLC ensure quality control and reproducibility. Overall, Tecoma stans shows promising potential as a standardized herbal formulation for effective diabetic wound healing.
Keywords: Tecoma stans, wound healing, diabetic ulcers, phytochemistry, herbal gel, antioxidant activity, HPLC, UV–Visible spectroscopy, analytical standardization, topical formulation
Cite This Article:
Please cite this article in press Mohammad Tausif Mohammad Anis et al., A Review On The Wound-Healing Potential And Analytical Standardization Of Tecoma Stans Ethanolic Gel, Indo Am. J. P. Sci, 2026; 13(02).
REFERENCES:
1. Guo S, DiPietro LA. Factors affecting wound healing. Journal of Dental Research. 2010;89(3):219–229.
2. Patel DK, Kumar R, Laloo D, Hemalatha S. Natural medicines from plant sources used for diabetic wound healing. Journal of Ethnopharmacology. 2012;144(2):266–277.
3. International Council for Harmonisation (ICH). ICH Harmonised Tripartite Guideline Q2(R1): Validation of Analytical Procedures—Text and Methodology. Geneva: ICH; 2005.
4. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound healing: A cellular perspective. Physiological Reviews. 2019;99(1):665–706.
5. Clark RAF. Biology of dermal wound repair. Dermatologic Clinics. 1993;11(4):647–666.
6. Eming SA, Krieg T, Davidson JM. Inflammation in wound repair: Molecular and cellular mechanisms. Journal of Investigative Dermatology. 2007;127(3):514–525.
7. Singer AJ, Clark RAF. Cutaneous wound healing. New England Journal of Medicine. 1999;341(10):738–746.
8. Baum CL, Arpey CJ. Normal cutaneous wound healing: Clinical correlation with cellular and molecular events. Dermatologic Surgery. 2005;31(6):674–686.
9. Falanga V. Wound healing and its impairment in diabetes mellitus. The Lancet. 2005;366(9498):1736–1743.
10. Bitar MS. Diabetes and impaired wound healing: Pathophysiology and therapeutic perspectives. International Journal of Biochemistry and Cell Biology. 2012;44(3):476–483.
11. Brem H, Tomic-Canic M. Cellular and molecular basis of wound healing in diabetes. Journal of Clinical Investigation. 2007;117(5):1219–1222.
12. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. Scientific World Journal. 2013;2013:162750.
13. Boots AW, Haenen GRMM, Bast A. Health effects of quercetin: From antioxidant to nutraceutical. European Journal of Pharmacology. 2008;585(2–3):325–337.
14. Middleton E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on inflammatory cells. Pharmacological Reviews. 2000;52(4):673–751.
15. Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999;12(4):564–582.
16. Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. Ethnopharmacological approaches to wound healing—Exploring medicinal plants of India. Journal of Ethnopharmacology. 2007;114(2):103–113.
17. Nayak BS, Sandiford S, Maxwell A. Evaluation of wound-healing activity of ethanolic extract of medicinal plants. Evidence-Based Complementary and Alternative Medicine. 2009;6(3):351–356.
18. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998.
19. Trease GE, Evans WC. Pharmacognosy. 16th ed. London: Saunders Elsevier; 2009.
20. Snyder LR, Kirkland JJ, Dolan JW. Introduction to Modern Liquid Chromatography. 3rd ed. Hoboken: John Wiley & Sons; 2010.
21. Skoog DA, Holler FJ, Crouch SR. Principles of Instrumental Analysis. 6th ed. Belmont: Thomson Brooks/Cole; 2007.
22. Gupta VK, Jain R, Mishra A, et al. Antioxidant and anti-inflammatory potential of medicinal plants. Pharmacognosy Reviews. 2010;4(8):117–126.
23. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. LWT – Food Science and Technology. 1995;28(1):25–30.
24. Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin and natural phytochemicals. International Journal of Biochemistry and Cell Biology. 2009;41(1):40–59.
25. Nostro A, Germano MP, D’Angelo V, Marino A, Cannatelli MA. Extraction methods and antimicrobial activity of medicinal plants. Letters in Applied Microbiology. 2000;30(5):379–384.
26. Modak M, Dixit P, Londhe J, Ghaskadbi S, Devasagayam TPA. Indian herbs and antidiabetic potential. Journal of Clinical Biochemistry and Nutrition. 2007;40(3):163–173.
27. Murthy S, Gautam MK, Goel S, et al. Evaluation of wound healing activity of plant extracts. International Journal of Low Extremity Wounds. 2013;12(2):91–99.
28. Aulton ME, Taylor KMG. Aulton’s Pharmaceutics: The Design and Manufacture of Medicines. 5th ed. London: Churchill Livingstone; 2018.
29. Allen LV. Pharmaceutical Dosage Forms and Drug Delivery Systems. 10th ed. Philadelphia: Lippincott Williams & Wilkins; 2014.
30. Rowe RC, Sheskey PJ, Quinn ME. Handbook of Pharmaceutical Excipients. 6th ed. London: Pharmaceutical Press; 2009.
31. Kalia YN, Guy RH. Modeling transdermal drug release. Advanced Drug Delivery Reviews. 2001;48(2–3):159–172.
32. World Health Organization. Quality Control Methods for Herbal Materials. Geneva: WHO Press; 2011.
33. Kunle OF, Egharevba HO, Ahmadu PO. Standardization of herbal medicines—A review. International Journal of Biodiversity and Conservation. 2012;4(3):101–112.
34. Dong MW. Modern HPLC for Practicing Scientists. Hoboken: John Wiley & Sons; 2006.
35. International Council for Harmonisation (ICH). ICH Guideline Q2(R2): Validation of Analytical Procedures. Geneva: ICH; 2022.
36. Shrivastava A, Gupta VB. Methods for determination of LOD and LOQ. Chronicles of Young Scientists. 2011;2(1):21–25.
37. Blessy M, Patel RD, Prajapati PN, Agrawal YK. Development of stability-indicating methods. Journal of Pharmaceutical Analysis. 2014;4(3):159–165.




