Volume : 11, Issue : 12, December – 2024
Title:
NOVEL APPROACH FOR ESTIMATION OF DRUG IN BIOLOGICAL SAMPLE RECENT TRENDS AND FUTURE PROSPECT
Authors :
Shubhada V. Bhopale*, Dr. Mahesh P. Kumar, Dr. Sunil N. kshirsagar
Abstract :
Accurate estimation of drugs in biological matrices is fundamental to pharmaceutical development, clinical therapeutics, toxicological evaluation, and regulatory compliance. The increasing complexity of modern drug molecules and the demand for ultra-trace detection have driven significant advancements in bioanalytical methodologies. Conventional techniques such as UV spectroscopy and high-performance liquid chromatography have progressively evolved into highly sensitive and selective hyphenated systems, particularly liquid chromatography–tandem mass spectrometry, which is now considered the gold standard in bioanalysis. Emerging technologies including high-resolution mass spectrometry, microfluidic platforms, nanotechnology-assisted detection, biosensors, and miniaturized sampling strategies such as dried blood spot and volumetric absorptive microsampling have further transformed the analytical landscape. In parallel, novel sample preparation approaches and green analytical methodologies have improved efficiency while reducing environmental impact. These innovations collectively address challenges associated with matrix interference, low analyte concentration, and high-throughput requirements. This review critically discusses recent advancements in drug estimation techniques, their applications in pharmacokinetics, therapeutic drug monitoring, toxicology, forensic science, and anti-doping analysis, and explores future perspectives emphasizing automation, artificial intelligence integration, and sustainable bioanalytical practices.
Keywords:Bioanalytical methods; LC–MS/MS; high-resolution mass spectrometry; pharmacokinetics; therapeutic drug monitoring; biosensors; microfluidics; nanotechnology; dried blood spot; green analytical chemistry; sample preparation; drug quantification.
Cite This Article:
Please cite this article in press Shubhada V. Bhopale et al., Novel Approach For Estimation Of Drug In Biological Sample Recent Trends And Future Prospect .,Indo Am. J. P. Sci, 2024; 11 (12).
Number of Downloads : 10
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