Volume : 13, Issue : 03, March – 2026

Title:

DESIGN, DEVELOPMENT AND EVALUATION OF EUPHORBIA HIRTA L. HAIR FORMULATION FOR HAIR GROWTH PROMOTION ON MICE

Authors :

P.Seeta* , J.N.Suresh Kumar , Sk. Riyaz , Ch. Siva Sai , G. Mounika , K. Sunil Kumar and Sk. Thaslima

Hair loss (alopecia) is a common dermatological condition that causes significant psychological stress. This study aimed to design, develop, and evaluate a herbal hair oil formulation using Euphorbia hirta L. (Euphorbiaceae) for hair growth promotion. An ethanolic extract of the whole plant was prepared via maceration and screened for phytochemicals, revealing the presence of flavonoids, tannins, terpenoids, and phenols. A stable herbal oil was formulated by incorporating 2% Euphorbia hirta extract into a coconut oil base using Tween 80 as a surfactant. The hair growth-promoting activity was evaluated in vivo using testosterone-induced alopecia models in Wistar mice. Four groups were studied over 30 days: a control group (sterilized water), a negative control (testosterone-induced), a positive control (0.1 mL of 5% finasteride), and a test group (0.8 mL of 10% Euphorbia hirta oil). Preliminary results suggest that the Euphorbia hirta oil formulation provides a promising natural alternative to synthetic drugs for treating hair growth disorders.
Keywords: Hair Growth Promotion, Alopecia, Herbal Formulation, Phytochemical Screening, Testosterone-Induced Alopecia, Wistar Mice, Flavonoids.

Cite This Article:

Please cite this article in press P.Seeta et al., Design, development and evaluation of euphorbia hirta l. Hair formulation for hair growth promotion on mice., Indo Am. J. P. Sci, 2026; 13(03).

REFERENCES:

1. Sharma PV. History of Ayurveda. Varanasi: Chaukhamba Orientalia; 1996.
2. Lele RD. Ayurveda and modern medicine. J Assoc Physicians India. 1999;47(6):625-628.
3. Kamboj VP. Herbal medicine. Curr Sci. 2000;78(1):35-39.
4. Alves RRN, Rosa IL. Biodiversity, traditional medicine and public health: where do they meet? J Ethnobiology Ethnomed. 2007; 3:14.
5. Dwyer JT, Rattray JB. Plant-derived medicinal agents and their role in therapy. Phytotherapy Res. 1993;7(3):195-201.
6. Cragg GM, Newman DJ, Snader KM. Natural products in drug discovery and development. J Nat Prod. 1997;60(1):52-60.
7. Harvey AL. Medicines from nature: are natural products still relevant to drug discovery? Trends Pharmacology Sci. 1999;20(5):196-198.
8. Millar SE. Molecular mechanisms regulating hair follicle development. J Invest Dermatol. 2002;118(2):216-225.
9. Liebich HG. Veterinary histology of domestic mammals. 4th ed. Oxford: Blackwell Publishing; 1993.
10. Schaefer H, Redelmeier TE. Skin barrier: principles of percutaneous absorption. Basel: Karger; 1996.
11. Saitoh M, Uzuka M, Sakamoto M. Human hair cycle. J Invest Dermatol. 1969;54(1):65-81.
12. Sharma RK. Cosmetic formulation and evaluation. New Delhi: CBS Publishers; 1998.
13. Waldman A, Brown M. Development and distribution of hair follicles. Dermatology. 1974;148(1):21-27.
14. Chase HB. Growth of the hair. Physiol Rev. 1954;34(1):113-126.
15. Stenn KS, Paus R. Controls of hair follicle cycling. Physiol Rev. 2001;81(1):449-494.
16. Olsen EA. Disorders of hair growth: diagnosis and treatment. Dermatol Clin. 1993;11(1):1-11.
17. Fitzpatrick TB, Eisen AZ, Wolff K, Freedberg IM, Austen KF. Dermatology in general medicine. 4th ed. New York: McGraw-Hill; 1993.
18. Dawber RPR, Van Neste D. Hair and scalp disorders. London: Martin Dunitz Ltd; 1998.
19. Barahmani N, Schabath MB, Duvic M. History of alopecia areata. Clin Dermatol. 2009;27(5):493-497.
20. Finner AM. Alopecia areata: clinical presentation, diagnosis and unusual cases. Dermatol Ther. 2011;24(3):348-354.
21. Dhanashri D. Jawarkar, Hair growth promoting activity of helianthus annuus in testosterone induced alopecia in mice model, Int. Journal of Pharmaceutical Sciences and Medicine (IJPSM), Vol.8 Issue. 5, May- 2023, pg. 80-91.
22. Banan A. Mohammed, Hair Growth Stimulation in Testosterone-Induced Alopecic Mice: A Comparative Study between Topical Clove Oil 10% Topical Minoxidil 5%, Journal of the Faculty of Medicine Baghdad Print ISSN: 0041-9419, Online ISSN: 2410-8057.