Volume : 13, Issue : 05, May – 2026
Title:
FORMULATION AND EVALUATION OF HERBAL MOUTHWASH CONTAINING NEEM (AZADIRACHTA INDICA) & TULSI (OCIMUM SANCTUM)
Authors :
Dr. R. H. Kale, Prof. A. S. Sontakke, Miss Sakshi Vishnu Shelke, Miss Sanika Sanjay Shirale, Miss Sakshi Rameshwar Wankhede, Miss Sanika Vitthal Ambhore, Miss Shubhangi Vijay Pophale
Abstract :
This study aims to formulate and evaluate a herbal mouthwash incorporating *Azadirachta indica* (neem) and *Ocimum sanctum* (tulsi) for routine oral hygiene practices. We collected neem and tulsi leaves, cleaned them, dried them, and then used water or hydro-alcoholic methods to create standardized plant extracts that were full of medicinal ingredients. We prepared a simple, alcohol-free mouthwash by mixing neem extract, tulsi extract, glycerin, peppermint oil, sodium benzoate (as a preservative), and distilled water to make a total of 100 mL. The formulation was tested for physical properties like color, clarity, smell, pH, and stability at room temperature for four weeks. The mouthwash’s pH was found to be in the mildly acidic to near-neutral range, which means it is safe to use in the mouth and is not likely to irritate the mucosa. The herbal solution was quite clear, and during the stability test, there was no phase separation or growth of microorganisms. The agar-well diffusion method was used to test the antibacterial activity against several bacterial strains that are often connected to oral diseases. The neem-tulsi mouthwash exhibited a distinct zone of inhibition, indicating significant antibacterial effectiveness. The results show that the herbal mouthwash that was made has good physicochemical properties and strong antibacterial properties, making it a safe and natural alternative to synthetic chemical mouthwashes for improving dental health. It is recommended to conduct further clinical trials and sensory evaluations to validate its acceptance and enduring efficacy.
Keywords: Herbal mouthwash, Neem (Azadirachta indica), Tulsi (Ocimum sanctum), Oral hygiene, Antimicrobial activity, Natural formulation.
Cite This Article:
Please cite this article in press R. H. Kale et al., Formulation And Evaluation Of Herbal Mouthwash Containing Neem (Azadirachta Indica) & Tulsi (Ocimum Sanctum)., Indo Am. J. P. Sci, 2026; 13(05).
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