Volume : 13, Issue : 05, May – 2026
Title:
DEVELOPMENT AND EVALUATION OF ANTI-INFLAMMATORY GEL FORMULATION FROM MIMOSA PUDICA LINN WITH CYTOTOXICITY SCREENING VIA BSLA
Authors :
Shankar Musale*, Aniket Kumbhar, Nagesh Kachare, Subhash Patil, Ritesh Patel, Samarth Kodag
Abstract :
The present study focused on the development and evaluation of an anti-inflammatory gel formulated from Mimosa pudica Linn. extract and perform Brine Shrimp Lethality Assay (BSLA) for cytotoxicity screening. The plant extract was prepared by maceration extraction method using methanol as solvent and perform preliminary phytochemical screening, which confirmed the presence of alkaloids, carbohydrates, flavonoids, tannins, steroids. To perform protein denaturation assay at different concentrations (100–500 µg/mL) for evaluation of anti-inflammatory activity of the extract. The extract showed significant inhibition of protein denaturation with an IC₅₀ value of around 220 µg/mL and represent good anti-inflammatory potential. Cytotoxicity screening using Brine Shrimp Lethality Assay (BSLA) demonstrated moderate biological activity, with 100% lethality at 1000 µg/mL and LC₅₀ value of 100 µg/mL. Herbal gel was formulated using the different conc. of Carbopol and evaluated for pH, viscosity, Spreadability, washability, transparency and extrudability. MPG F4 batch showed optimum physicochemical properties and was selected for optimized formulation. The study recommends that Mimosa pudica Linn. gel is useful and effective topical formulation for inflammation management.
Keywords: Mimosa pudica L., Anti-inflammatory gel, Brine Shrimp Lethality Assay (BSLA), Cytotoxicity, protein denaturation assay.
Cite This Article:
Please cite this article in press Shankar Musale et al., Development And Evaluation Of Anti-Inflammatory Gel Formulation From Mimosa Pudica Linn With Cytotoxicity Screening Via Bsla.., Indo Am. J. P. Sci, 2026; 13(05).
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