Volume : 13, Issue : 06, June – 2026
Title:
IN VITRO EVALUATION OF THE ANTI-CANCER, ANTI-DIABETIC, AND ANTI-INFLAMMATORY ACTIVITIES OF ETHANOLIC EXTRACT OF ZINGIBER OFFICINALE
Authors :
Bhosale Shivaji Suryakant
Abstract :
Background: Plant-derived therapeutic agents represent a key reservoir for novel safe, economical, and biocompatible drugs. Zingiber officinale (ginger) is widely used across traditional medicine frameworks due to its vast collection of bioactive secondary metabolites. This research systematically evaluates the multi-targeted therapeutic potential of the ethanolic extract of Zingiber officinale rhizome using customized in vitro screening protocols.
Methods: High-efficiency extraction was conducted utilizing a Soxhlet apparatus with ethanol as the processing solvent. Cytotoxic profiling against established human cancer cell lines was measured using the classic MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay across a range of concentrations. Postprandial anti-diabetic potential was investigated through an in vitro a-amylase enzyme inhibitory test matrix coupled with a colorimetric dinitrosalicylic acid (DNS) system. The protective anti-inflammatory efficacy was evaluated via an optimized bovine serum albumin (BSA) heat-induced protein denaturation prevention assay.
Results: The experimental outcomes revealed a definitive dose-dependent mechanism across all three experimental lines. The ethanolic extract caused significant growth inhibition and structural shrinkage of cancer cells, translating to a stark down-regulation in overall cell viability. In parallel, the extract demonstrated a powerful capacity to inhibit a-amylase activity, thereby establishing a valid pathway for controlling carbohydrate breakdown. Furthermore, a substantial percentage inhibition of heat-mediated protein denaturation was observed, showing therapeutic efficacy comparable to standard reference anti-inflammatory pharmaceutical agents.
Conclusion: These data demonstrate that the ethanolic extract of Zingiber officinale possesses significant anti-cancer, anti-diabetic, and anti-inflammatory properties driven by its abundant phenolic, flavonoid, and gingerol contents. These findings serve as an important baseline validating further isolation trials and in vivo validation models.
Keywords: Zingiber officinale, Ethanolic Extract, MTT Assay, a-Amylase Inhibition, Protein Denaturation, Apoptosis.
Cite This Article:
Please cite this article in Bhosale Shivaji Suryakant., In Vitro Evaluation Of The Anti-Cancer, Anti-Diabetic, And Anti-Inflammatory Activities Of Ethanolic Extract Of Zingiber Officinale, Indo Am. J. P. Sci, 2026; 13(06).
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