02.Issue 09 september 26 - INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES

Volume : 13, Issue : 09, September – 2026

Title:

A NEW ANALYTICAL RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS DETERMINATION OF NIRMATRELVIR AND RITONAVIR IN BULK FORM AND PHARMACEUTICAL DOSAGE FORM

Authors :

G. Vijaya Kumar*, B. Sravanthi, Gaini Pavan

Abstract :

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Nirmatrelvir and Ritonavir in pure form and marketed combined pharmaceutical dosage forms. A column having Symmetry (C18) (150mm x 4.6mm, 5µm) in isocratic mode with mobile phase containing Methanol: Phosphate Buffer (pH-3.8) (28:72v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 252 nm. The retention time (min) and linearity range (ppm) for Nirmatrelvir and Ritonavir were (1.788, 3.475min) and (10-30, 10-50), respectively. The method has been validated for linearity, accuracy and precision, robustness and limit of detection and limit of quantitation. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.86µg/ml and 2.58µg/ml for Nirmatrelvir and 1.28µg/ml 3.84µg/ml for Ritonavir respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of Nirmatrelvir and Ritonavir in tablets.
Keywords: Nirmatrelvir and Ritonavir, RP-HPLC, Validation, Accuracy, Precision.

Cite This Article:

Please cite this article in press G. Vijaya Kumar et al., A New Analytical Rp-Hplc Method Development And Validation For The Simultaneous Determination Of Nirmatrelvir And Ritonavir In Bulk Form And Pharmaceutical Dosage Form.,. Indo Am. J. P. Sci, 2026; 13(09).

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