Volume : 13, Issue : 09, September – 2026

Title:

FORMULATION AND EVALUATION OF GINKGO BILOBA EXTRACT LOADED PHYTOSOMES FOR NEUROPROTECTIVE ACTIVITY

Authors :

K. Chaitanya Sravanthi*, Sabavath Swetha, Yaswitha Thati, Mandala Harika, Sania Anjum, Narapogu Shirisha

Abstract :

Ginkgo biloba is a well-known medicinal plant possessing antioxidant and neuroprotective properties, but its therapeutic efficacy is limited due to poor bioavailability and low aqueous solubility. The present study was aimed at formulating and evaluating Ginkgo biloba extract loaded phytosomes to enhance its bioavailability and therapeutic efficacy. Phytosomes were prepared by the solvent evaporation method using phosphatidylcholine as the phospholipid carrier for the formation of stable phyto-phospholipid complexes.The prepared formulations were evaluated for particle size, zeta potential, entrapment efficiency, surface morphology, in-vitro drug release, and stability studies. FTIR analysis confirmed the successful formation of phytosomal complexes between the extract and phospholipids. SEM analysis revealed spherical vesicles with smooth surface morphology. Among all formulations, F4 exhibited the smallest particle size of 244 nm and highest entrapment efficiency of 85.26%, indicating improved stability and drug incorporation.In-vitro drug release studies demonstrated sustained release behavior over a period of 8 hours, with maximum drug release of 98.88% observed for formulation F4. Stability studies carried out at different storage conditions showed no significant changes in drug release and formulation characteristics, confirming the stability of the optimized phytosomal formulation. The study concluded that Ginkgo biloba extract loaded phytosomes could serve as a promising delivery system for improving bioavailability, sustained drug release, and neuroprotective activity.
Keywords: Ginkgo biloba extract, FTIR Studies, Solvent evaporation method, In vitro drug release studies

Cite This Article:

Please cite this article in press K Chaitanya Sravanthi et al., Formulation And Evaluation Of Ginkgo Biloba Extract Loaded Phytosomes For Neuroprotective Activity.,. Indo Am. J. P. Sci, 2026; 13(09).

REFERENCES:

1. Karunamoorthi K, Jegajeevanram K, Jerome X, Vijayalakshmi J,Melita L. Tamil traditional medicinal system – Siddha: an indigenous health practice in the international perspectives. International journal of Genuine Traditional Medicine. 2012, Vol: 2(2):1- 11.
2. Legal status of traditional medicine and complementary/ alternative medicine: a worldwide review (document who/edm/trm/2001.2). Geneva, world health organization, 2001
3. Dawit A. Traditional medicine in Ethiopia: the attempt being made to promote it for effective and better utilization. SINET: Ethiopian Journal of Sciences. 1986, Vol: 9(4):61-69.
4. Kong JM, Goh NK, Chia LS, Chia TF. Recent advances in traditional plant drugs and orchids. Acta Pharmacol Sin. 2003,Vol: 4(2):7-21.
5. Joy PP, Thomas J, Mathew S, Skaria BP. Medicinal plants. In: Bose TK, Kabir J, Joy PP, ed. Tropical Horticulture. Kolkata, India, 2001,vol: 2(4):1-12.
6. Hamsaveni Gopal, Saraswathi Sukumar and Purushotaman K.K. Antimalarials from Indian medicinal plants. Journal. Res in Ayurveda Siddha., 1981,Vol: 2 (3): 286- 295.
7. Ravishankar, B And Shukla, Indian Systems Of Medicine: A Brief Profile Complementary And Alternative Medicines V.J. African. Journal of Traditional medicine. Cam (2007), vol:4 (3): 319 – 337.
8. Qazi majaz A, Molvi khurshid I., Hserbal medicine: a comprehensive review. international journal of pharmaceutical research, Apr – June 2016, Vol: 8(2) 1-5.
9. World Health Organization. Traditional Medicine Strategy 2002-2005 (WHO/EDM/TRM/2002.1). Geneva, Switzerland: World Health Organization, 2002.
10. Imam Hashmat , Hussain Azad and Ajij Ahmed Neem (Azadirachta indica A. Juss) – A
Nature’s Drugstore: An overview. International Research Journal of Biological Sciences. October (2012), Vol:1(6):76-79.
11. Lele R D. Ayurveda (Ancient Indian System of Medicine) and modern molecular medicine. Joural of the Asian of Physicians India.1999,vol: 1(2):625-685.
12. Tlxx-Liwes, F. and W. Beyne, Dosage form index; an objective crilerion for evaliiiition of controlled release drug delivery systems, J.Pharm.Sci. 66, 13S.S(I977).
13. Brandau R., Lippoid, B. (Eds.) “Dermal and Transdermal Absorption”, 1st International Symposium”, Munich, 1981. pp. 171-173.
14. Jain NK. Controlled and Novel Drug Delivery. 1st ed. New Delhi: CBS Publisher and Distributors; 2001. p. 100-29.
15. Darwhekar G, Jain DK, Paditar VK. Formulation and evaluation of transdermal drug delivery system of clopidogrel bisulfate. Asian J Pharm Life Sci 2011;1:269-78.
16. Shingade GM, Aamer Q, Sabale PM, Grampurohit ND, Gadhave MV. Review on: Recent trend on transdermal drug delivery system. J Drug Deliv Ther 2012;2:66.