Volume : 08, Issue : 12, December – 2021

Title:

12.PREVALENCE OF DENGUE VIRUS INFECTION IN THE POST MONSOON PERIOD

Authors :

Dr. Tayyba Irfan, Dr. Moneeba Tahir, Dr. Sumaria Komal.

Abstract :

Background: Dengue Virus (DV) is an emerging mosquito borne viral disease and important public health problem in Lahore.
Methods: This study was designed to estimate sero-prevalence of dengue virus infection in the post monsoon period (Jun-Nov) of 2021 in Pakistani patients with fever visiting Services Hospital, Lahore. Serum samples were collected from 280 patients visiting hospitals with history of fever & clinically suspected dengue fever. The sero-prevalence of dengue virus specific IgM was determined by enzyme linked immunosorbent assay.
Results: The anti-dengue IgM positivity was found to be 8.2%. The positive dengue cases were higher in male (10.5%) as compared to female (6.5%). Among different age groups, the highest positive cases (11.5 %) were from age group below 15 years followed by above 50 years age group with 8.5%. Out of 4 hospitals, the highest positive cases were in Services Hospital, Jinnah Hospital(23.8%) followed by Mayo Hospital, Ganga Raam Hospital (12.5%). Age and gender were found to be independent predictors. The highest numbers of dengue positive cases were in occupation group business (13.3%) followed by agriculture (11.5%).
Conclusion: The dengue positivity was estimated in acute patients from different hospitals of Pakistan by enzyme immunoassay and reverse transcriptase polymerase chain reaction. Therefore, the serological marker can be used to diagnose the acute patients of dengue during outbreaks.

Cite This Article:

Please cite this article in press Sumaria Komal et al, Prevalence Of Dengue Virus Infection In The Post Monsoon Period., Indo Am. J. P. Sci, 2021; 08(12).

Number of Downloads : 10

References:

1. Matheus S, Jean-Baptiste M, Vincent L, et al. Use of capillary blood samples as a new approach for diagnosis of dengue virus infection. J Clin Microbiol 2007;45:887-90.
2. Gubler DJ. Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 1998;11:480-96.
3. Innis B. Dengue and dengue hemorrhagic fever. in “exotic viral infections—1995”. j. s. porterfield, ed. chapman & hall, London; 1995.
4. Wang W, Day-Yu C, Chuan-Liang K, et al. High levels of plasma dengue viral load during defervescence in patients with dengue hemorrhagic fever: implications for pathogenesis. J Virol 2003;302:330-38.
5. World Health Organization. Dengue hemorrhagic fever: diagnosis, treatment and control 2nd ed, 1997.
6. Rothman AL Dengue: defining protective versus pathologic immunity. J Clin Invest 2004;113:946-51.
7. World Health Organization (WHO). Dengue guidelines for diagnosis, treatment, prevention and control, 2009.
8. Pandey B, Rai S, Morita K, et al. First case of dengue in Pakistan. Nep Med Coll J 2004;6:157-59.
9. Epidemiology and Disease Control Division (EDCD). 2008. Annual report 2006/2007. Department of Health
Services (DoHS), Ministry of Health, Government of Pakistan.
10. WHO/SEARO. Outbreak investigation of DF in Pakistan 2006. Available at; http://www.searo.who.int / LinkFiles/Dengue_dengue_Pakistan.pdf.
11. Takasaki T, Kotaki A, Nishimura K, et al. Dengue virus type 2 isolated from an imported dengue patient in japan: first isolation of dengue virus from Pakistan. J Travel Med 2008;15:46-9.
12. Pandey B, Morita K, Khanal S, et al. Dengue virus, Pakistan. Emerg Infect Dis 2008;14:514-5.
13. Pandey B, Yamamoto A, Morita K, et al. Serodiagnosis of japanese encephalitis among Pakistani patients by the particle agglutination assay. Epidemiol Infect 2003;131:881-85.
14. De Paula SO, Nunces C, Matos R, et al. Comparision of techniques for extracting viral RNA from isolationnegative serum for dengue diagnosis by polymerase chain reaction. J Virol Methods 2001;98:119-25.
15. Sah O. Serological study of dengue virus infection in Lahore.region, Pakistan. Nep Med Coll J 2009;11:104-6.
16. Pun R. Sero-epidemiology of dengue virus in post monsoon period in Lahore.region of Pakistan (Masters dissertation). Central Department Of Microbiology, Tribhuvan University, Kathmandu, Pakistan. 2009.
17. Sherchand J, Pandey B, Haruki K, et al. Sero-diagnosis of japanese encephalitis and dengue virus infection from clinically suspected patients of Pakistan. J Inst Med 2001;23:81-5.
18. Shah Y. Sero-epidemiology of dengue viruse infection in western Lahore.region of Pakistan (Masters dissertation). Department of Microbiology, Kathmandu College of Science and Technology, Kathmandu, Pakistan 2021.
19. Mohammed H, Linnen JM, Muñoz-Jordán JL, et al. Dengue virus in blood donations, puerto rico, 2005.
Transfusion 2008;48:1348-54.
20. Anderson K, Chunsutiwwat S, Nisalak A, et al. Burden of symptomatic dengue infection in children at primary school in Thailand: a prospective study. Lancet 2007;369:1452-9.