Volume : 09, Issue : 03, March – 2021
10.COMPARATIVE ANALYSIS OF ANTIBIOTIC SUSCEPTIBILITY AMONG BETA-LACTAMASE PRODUCING STRAINS FROM DIFFERENT CLINICAL SAMPLES IN PAKISTAN, LAHORE
Fadia Waheed, Dr. Saba Riaz, Muhammad Farhan Zafar Chaudhry
Abstract :
Increasing trend of antimicrobial drug resistance in extended spectrum beta-lactamase (ESBLs) producing bacteria is one of the major problems to the health sectors these days. Most of the bacteria produce beta-lactamase enzymes which inactivate the beta-lactam ring of the antibiotics and become resistant to that drug. The aim of the study was the comparative analysis of antibiotic susceptibility among beta-lactamase producing strains from different clinical samples in Pakistan, Lahore. The present study was conducted at Citi Lab and Research Centre. All specimens received in the lab were processed according to Clinical Laboratory Standard Institute (CLSI, 2006). Identification of strains was performed using biochemical battery and analytical profile index. ESBLs were phenotypically examined by antibiotic susceptibility (AST) test, double disk synergism (DDST) and combination disk synergy test (CDST). Out of 300 strains isolated from different clinical samples (urine, wound and blood) 157 isolates belonged to Enterobacteriaceae family. Phenotypic analysis showed 59/157(37.5%) were ESBL producing strains. These strains include E. coli, Klebsiella pneumonia, Morganella morganii, Citrobacter spp, Proteus spp. Urine revealed 41% ESBLs producers following wound 16%, blood 2%. Data presents that ESBLs producers were predominant in the females as compared to males following age variation groups, vary from area to area, gender variation in health care facilities. Amikacin, Tazobactams and Sulzone are still the drugs of choice for such infections. Proper antibiogram profile evaluation is very significant for managing such infection.
Key words: Enterobacteriaceae, Extended spectrum beta-lactamases, Susceptibility, AST, DDST, CDST.
Cite This Article:
Please cite this article in press FadiaWaheedet al.,Comparative Analysis Of Antibiotic Susceptibility Among Beta-Lactamase Producing Strains From Different Clinical Samples In Pakistan, Lahore.,Indo Am. J. P. Sci, 2021; 08(03).
Number of Downloads : 10
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