Volume : 08, Issue : 05, May – 2021
Title:
13.SIMPLE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF METHOTREXATE AND CURCUMIN IN BULK DRUGS
Authors :
Ayesha Syed and *and V. Kusum Devi
Abstract :
A method of estimation for the simultaneous estimation of methotrexate and curcumin in their bulk powder is developed and validated as per ICH guidelines. The method involved measurement of absorbance of the methotrexate and curcumin at the 𝜆max of the drugs i.e., 307nm and 425nm in methanol. Calibration curves of methotrexate and curcumin were found to be linear in the range 2-18µg/mL and 1-7 µg/mL respectively. The correlation coefficient values R2 was found to be 0.999. LOD and LOQ were 122.23ng/mL and 370.4ng/mL for methotrexate, 12.69ng/mL and 38.48ng/mL for curcumin. The percentage recovery at various concentration levels varied in between 99 to 101% for both the drugs estimated, thereby confirming accuracy of the method developed. In the precision study, the %RSD was found to be less than 2%, indicating the method is precise. Hence it can be concluded that the method developed is simple, accurate, precise, and economical.
Keywords:- Methotrexate, Curcumin, Simultaneous estimation and Validation.
Cite This Article:
Please cite this article in press Ayesha Syed and V. Kusum Devi., Simple Spectrophotometric Method For Simultaneous Estimation Of Methotrexate And Curcumin In Bulk Drugs., Indo Am. J. P. Sci, 2021; 08(05).
Number of Downloads : 10
References:
1. T.M. Wyeth Pfizer Canada Inc. Product Monography: Pr METHOTREXATE
2. Shinde G C, Venkatesh M.P, kumar Pramod TM and Shivkumar G H. Methotrexate: A Gold Standard for Treatment of Rheumatoid Arthritis. Journal of Pain & Palliative Care Pharmacotherapy. 2014; 28:351–58.
3. Sharma RA, Gescher AJ, Steward WP. Curcumin: The story so far. Eur J Can. 2005; 41(13): 1955-1968.
4. Yadav VR, Prasad S, Kannappan R, Ravindran J, Chaturvedi MM, Vaahtera L, Parkkinen J, Aggarwal BB. Cyclodexrin-complexed curcumin exhibits antiinflammatory and antiproliferative activities superior to those of curcumin through higher cellular uptake. Biochem Pharmacol. 2010; 80(7): 1021-1032.
5. Banji D, Pinnapureddy J, Banji FJO, Kumar RA, and Reddy NK. Evaluation of the concomitant use of methotrexate and curcumin on Freund’s complete adjuvant induce arthritis and hematological indices in rat. Indian J Pharmacol. 2011 Sep¬OctÍľ 43(5): 546–550
6. Moghadam RA, Tutunchi S, Abad ANA, Yazdi M, Bonyadi F, Mohajeri D et al. Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress. BMC Complementary and Alternative Medicine (2015) 15:246
7. Mariann Garner-Wizard et al. Comparative Study of Curcumin and Diclofenac Sodium in Rheumatoid Arthritis Patients. HerbClip. 2012.
8. Thangapazham RL, Sharma A, Maheshwari RK. Multiple molecular targets in cancer chemoprevention by curcumin. The AAPS J. 2006; 8(3):E443-E449