Volume : 08, Issue : 11, November – 2021
Title:
12.HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF ITRACONAZOLE AND TERBINAFINE IN BULK AND PHARMACEUTICAL DOSAGE FORM
Authors :
G. Indira Priyadarshini* Manchi venkata Chari
Abstract :
A simple, Accurate, precise method was developed for the simultaneous estimation of the Terbinafine and Itraconazole in bulk and pharmaceutical dosage form. Chromatogram was run through phenomenex C18 150 x 4.6mm, 5.0m. Mobile phase containing Buffer 0.1%OPA: Methanol taken in the ratio 60:40v/v was pumped through column at a flow rate of 1.0ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 245 nm. Retention time of Terbinafine and Itraconazole were found to be 2.331 min and 2.934 min. %RSD of the Terbinafine and Itraconazole were and found to be 0.4 and 0.9 respectively. %Recovery was obtained as 99.95% and 100.98% for Terbinafine and Itraconazole respectively. LOD, LOQ values obtained from regression equations of Terbinafine and Itraconazole were 0.94, 2.86 and 0.70, 2.13 respectively. Regression equation of Terbinafine is y = 6951.x + 2494., and y = 7657x + 2075. of Itraconazole Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
Key Words: Terbinafine, Itraconazole, RP-HPLC
Cite This Article:
Please cite this article in press G. Indira Priyadarshini et al, HPLC Method Development And Validation For The Simultaneous Estimation Of Itraconazole And Terbinafine In Bulk And Pharmaceutical Dosage Form
., Indo Am. J. P. Sci, 2021; 08(11).
Number of Downloads : 10
References:
1. R. S. Satoskar, S. D. Bhandarkar and S. S. Ainapure. “Pharmacology and Pharmacotherapeutics”, 17th edition, Popular Prakashan, Mumbai, India, 2001.
2. “Burger’s Medicinal Chemistry and drug discovery”, 6 th edition, Wiley Interscience, New Jersey, 2007.
3. “Wilson and Gisvold’s Textbook of Organic Medicinal and Pharmaceutical Chemistry”, 11th edition, Lippincott Williams & Wilkins, New york, 2004.
4. Korolkovas. “Essentials of Medicinal Chemistry”, 2nd edition, Wiley Interscience, New Jersey, 1988.
5. “Goodman and Gilman’s The Pharmacological Basis of Therapeutics”, 9th edition, McGraw-Hill health professions division, New york, 1996.
6. Foye’s “Principles of Medicinal Chemistry”, 6th edition, Lippincott Williams & Wilkins, New york, 2008.
7. Drugs & Cosmetics Act, 1940 & Rules, 1945, 2nd edition, Susmit publishers, Mumbai, India, 2000.
8. Indian Pharmacopoeia, Ministry of Health & Family Welfare, Government of India, New Delhi, 1996.
9. The United States Pharmacopoeia- the National Formulary, United States Pharmacopoeial convention, Rockville, 2007.
10. British Pharmacopoeia, The Stationary Office, London, 2005.
11. “Martindale – The Extra Pharmacopoeia”, 33rd edition, The Pharmaceutical Press, London, 2002. 7
12. H. Beckett and J. B. Stenlake. “Practical Pharmaceutical Chemistry”, Volume I and II, CBS Publishers & Distributors, New Delhi, India, 2000.
13. P. D. Sethi. “Quantitative Analysis of Drugs in Pharmaceutical Formulations”. 3 rd edition, CBS Publishers & Distributors, New Delhi, India, 1997.
14. H. H. Willard, L. L. Merrit, J. A. Dean and F. A. Settle. “Instrumental Method of Analysis”, 7th edition, CBS Publishers & Distributors, New Delhi, India, 1986.
15. R. A. Day and A. L. Underwood. “Quantitative Analysis”, 6th edition, PHI learning private limited, New Delhi, India, 2009.