Volume : 08, Issue : 11, November – 2021



Authors :

Khushbu S. Patel, Dr.Sejal G. Patel and Dr. Richa Dayaramani

Abstract :

Antidiabetic drugs used in diabetes to treat diabetes mellitus by lowering the glucose level in the blood. Antidiabetic drugs mainly classify in oral hypoglycemic agent and insulin preparation. Where Anagliptin and Metformin HCl both drugs are oral hypoglycemic agents. In the Anagliptin is Dipeptidyl peptidase-4 inhibitors is also known as gliptins class and Metformin belong to bigunide, combination of Anagliptin and Metformin HCl give interactive effect and also homeostasis model assessment of Beta-index. Many Analytical Methods developed for single Metformin HCl & Anagliptin. Metformin is official in IP, BP, USP, and other methods like….UV, SPECTROPHOTOMETRIC, RP-HPLC, LC-MS,MS/MS are also developed. Anagliptin is not official in any pharmacopeia but ELISA, NMR, UV, LC/MS are developed. In combination of Anagliptin and Metformin HCl for first order derivative UV method is developed.
Key Words: Analytical Methods, Anagliptin, Metformin HCl

Cite This Article:

Please cite this article in press Khushbu S. Patelet al, Overviews on Analytical Methods of Anagliptin and Metformin Hydrochloride, Indo Am. J. P. Sci, 2021; 08(11).

Number of Downloads : 10


1] Shah S and Maheshwari D. First order derivative spectrophotometric method for simultaneous estimation of anagliptin and metformin HCl in bulk and synthetic mixture. J of Globle Trend in Pharmaceutical Sciences 2015; 6(3): 2925-29.
2] Bhatti P and Panchal H. Stability indicating chromatographic method development and validation for the simultaneous estimation of metformin HCl & anagliptin in its synthetic mixture by HPLC. World J of Pharma Research 2017; 6(11): 956-73.
3] Majithia R and Shah J.Development and validation of analytical method for estimation of Anagliptin tablet dosage form by UV Spectrophotometric method. International J of Pharmacy and Technologies 2015; 6: 7765-71.
4] Patil A and Shiekhedkar A. development and validation of five simple UV-Spectrophotometry methods for estimation of Anagliptin in bulk and in-house tablets. Research Gate 2016; 7(2): 127-131.
5] Pandya C and Rajput S. Stress degradation studies of anagliptin, development of validated stability indicating method, degradation kinetics study, identification and isolation of degradation products. Article in Chromatographia 2018; 1-21.
6] Brain S and Sigalapalli D. LC/MS/MS Characterization, molecular docking and in silico toxicity prediction studies on degradation products of Anagliptin. J of Pharma and Biomedical Analysis 2018; 159:92-99.
7] Arayne MS and Sultana N: Spectrophotometric Quantitation of Metformin in Bulk Drug and Pharmaceutical Formulation Using Multivariate Technique.Indian Journal Pharmaceutical Science2009; 71:331-335.
8] Umapathi P and Ayyappan J: Quantitative Determination of metformin hydrochloride in tablet formulation containing croscarmellose sodium as disintegrant by HPLC and UV spectrophotometry. Tropical Journal of Pharmaceutical Research 2012; 11 (1): 107-16.
9] Zhang W and Zhao H: Determination of Metformin in rat plasma by HILIC-MS/MS combined with Tecan automation and direct injection. Biomedical chromatography 2011.
10] Mubeen G and Khalikha N: Spectrophotometric Method for Estimation of Metformin Hydrochloride. International Journal Chemical Techanical Research 2010; 2: 1330-1331.
11] Reddy C and Mubeen G: HPTLC method for estimation of metformin hydrochloride. Biomed. & Pharmacol. Journal 2008; 1(2): 445-48.
12] Indian Pharmacopoeia, India: Government of India Ministry of health & family welfare and Published by Indian Pharmacopoeia Commission, Vol. II, 2014: 503, 728.
13] British Pharmacopoeia, London: Medicine & Healthcare Products Regulatory Agency, Vol. II, 2010: 3813-14.
14] United States Pharmacopoeia 30, National Formulary 25, Rockville: United States Pharmacopoeia Convention, Vol. II, 2010: 1092-93.
15] ICH Harmonized Tripartite Guideline, ICH Q2 (R1); Text & Methodology; October-1994.
16] Tripathi KD: Essential of Medical Pharmacology. Jaypee Brother Mesical Publishers Ltd, Sixth Edition 2008.
17] Ahuja S. and Scypinski S. 2001 Handbook of Modern Pharmaceutical Analysis.Academic Press, Second Edition; London: 356-367.
18] Sharma BK 2011: Instrumental Method of Chemical Analysis. Goel Publishing Housing, Krishna Prakashan Ltd, 27th edition: 68-175.
19] Mohan H: Textbook of Pathophysiology. Medical publishers Pvt Limited, Sixth Edition 2005.
20] Rang H. and Dale M: Range & Dale Pharmacology. Elsevier Publication Seventh Edition 2012.
21] https://go.drugbank.com/drugs/DB00331[Accessed 11 October 2021].
22] “Metformin Hydrochloride Compound” [Accessed 07 August 2021].
23] https://pubchem.ncbi.nlm.nih.gov/compound/44513473 [Accessed 21 July 2021].
24] https://www.sciencedirect.com/topics/medicine-and-dentistry/anagliptin [Accessed 21 July 2021].
25] https://www.diabetes.co.uk/diabetes-medication/dpp-4-inhibitors.html [Accessed 11 November 2021]