Volume : 09, Issue : 04, April – 2022
Title:
20.PREDICTING ACCELERATED RHEUMATOID ARTHRITIC INCIDANCE OF STATINS BY IN VITRO TECHNIQUES
Authors :
Jithin Mathew *, Litty Joseph, Sujitha P.V
Abstract :
Statins are the drugs used for the treatment of hyperlipidemia along with cholesterol lowering activity. In addition to cholesterol lowering activity these drugs may eventually leads to dysregulation of immune response. The primary objective of the present study focused on the induction of autoimmunity.The autoimmune response of statins is mediated by regulating leucocyte-endothelial cell adhesion, reducing nitric oxide production and promote the level of inflammatory cytokines such as tumour necrosis factor (TNF-α), interleukin 1 and interleukin 6. The dual effects of lipoprotein improvement and arthritic potential might be expected to confer an accelerated arthritic onset in patients with rheumatoid arthritis. The main study performed is denaturation of protein and inhibition of proteases assay. The result showed that pitavastatin and lovastatin administration during the active range of 62.5 – 500µg/ml indicated highest inhibitory profile on denaturation of proteins. The statins also exhibited maximum protease inhibitory activity during the percentage range of 12.28- 64.4% as compared to standard drug Diclofenac sodium. The recent evidence obtained from the study indicated both statins had little effect on accelerating rheumatoid arthritis. The study claimed that both Pitavastatin and lovastatin use promoted a significant role for developing rheumatoid arthritis.
Keywords: Pitavastatin, Lovastatin, Protein denaturation, Protease inhibition, Autoimmunity
Cite This Article:
Please cite this article in press Jithin Mathew et al, Predicting Accelerated Rheumatoid Arthritis Incidance Of Statins By In Vitro Techniques., Indo Am. J. P. Sci, 2022; 09(04).,
Number of Downloads : 10
References:
1. Kediri S Ben, Maid M, Baroda S, Molalla D, Shannon Z, Reba T. In Vivo Evaluation of the Anti-Inflammatory Effect of Pistachiomeniscus Fruit Oil and Its Effects on Oxidative Stress. 2016; 2016.
2. T. Sheela Rani, Annapoorna Vadivelu, Lakhshmi Devi, Ravi Teja, K. Chitra, C. Uma Maheswara Reddy. In Vitro Anti Arthritic Activity of Grewia tiliaefolia (Aerial parts). Research J. Pharm. and Tech. 4(12): Dec. 2011; Page 1833-1834.
3. Atul Ramchandra Chopade, Fahim Jehangir Sayyad. Evaluation of membrane stabilizing and inhibition of protein denaturation activity of Phyllanthus fraternus Webster. Research J. Pharm. and Tech. 6(3): March 2013; Page 251-254.
4. B. Thawkar, M. Kale, M. Oswal, K. Maniyar, K. Kadam, S. Kamat. To study Anti-inflammatory activity of 70% Methanolic Extract of Triumfetta rhomboidea : In Vitro Study. Research J. Pharm. and Tech. 9(3): Mar., 2016; Page 241-244.
5. Cojocaru L, Rusali AC, Cristina F, Mihaela A, Maria F, Craiu E. The Role of Simvastatin in the Therapeutic Approach of Rheumatoid Arthritis. 2013;2013.
6. Vandebriel RJ, Jong HJI De, Gremmer ER, Klungel OH, Tervaert JC, Slob W, et al. Statins accelerate the onset of collagen type II-induced arthritis in mice. Arthritis Res Ther [Internet]. 2012;14(2):R90. Available from: http://arthritis-research.com/content/14/2/R90.
7. B.S. Virupaxappa, K.H. Shivaprasad, M.S. Latha Swetha G.A.. Spectrophotometric Method for the Determination of Pitavastatin Calcium. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 643-645.
8. Vanita P. Rode, Madhukar R. Tajne. A Validated Stability-Indicating High-Performance Thin-Layer Chromatographic Method for the Analysis of Pitavastatin in Bulk Drug and Tablet Fomulation. Asian J. Pharm. Ana. 2018; 8(1): 49-52.
9. Sanjay Walode, Shailendra Gurav, Avinash Kasture. LC-ESI-MS/MS method for Quantitation of Lovastatin in Rat Plasma and Liver Homogenate: Application to Pharmacokinetic Study. Asian J. Research Chem. 7(10): October- 2014; Page 870-876.
10. P. Jitendra kumar, Y. Indira Muzib, Gitanjali Misra. Formulation and Evaluation of Pulsatile Drug Delivery of Lovastatin. Research J. Pharm. and Tech 2018; 11(7): 2797-2803.
11. Dhobale S, Narad V, Gaikwad D. Estimation of Lovastatin in Pharmaceutical Formulation by Area under Curve Spectrophotometric Method. 2017;6(3):85–93.
12. A. Anbarasi, R. Vidhya. Evaluation of In Vitro Anti- Inflammatory Activity of Tephrosia purpurea (Seed). Asian J. Pharm. Res. 5(2): April-June 2015; Page 83-89.
13. Thirumalai V, Nirmala P, Venkatanarayanan R. In vitro Anti-arthritic activity of Methanolic leaf extract of Cadaba indica Lam. Research J. Pharm. and Tech 2020; 13(3): 1219-1223.
14. A. Sureka, C. Mary Sharmila, R. Chithra Devi, N. J. Muthu Kumar, V. Banumathi. Evaluation of In Vitro Anti Inflammatory activity of Kusta Gaja Kesari – A Siddha Herbo Mineral Formulation against Albumin Protein Denaturation . Asian J. Pharm. Res. 2018; 8(3): 145-147.
15. Bijina B, Chellappan S, Krishna JG, Basheer SM, Elyas KK, Bahkali AH, et al. drug and as seafoof preservative Protease inhibitor from Moringa oleifera with potential for use as therapeutic drug and as seafood preservative. Saudi J Biol Sci [Internet]. 2011;18(3):273–81. Available from: http://dx.doi.org/10.1016/j.sjbs.2011.04.002.
16. Leelaprakash G, Dass SM, Road B. Available online http://www.ijddr.in Covered in Official Product of Elsevier , The Netherlands © 2010 IJDDR INVITRO ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF ENICOSTEMMAAXILLARE.2011;3(3):189–96.