Volume : 09, Issue : 08, August – 2022

Title:

25.STANDARDIZATION AND MICROSCOPICAL EVALUATION STUDIES OF CENTELLA ASIATICA LINN. (BRAHMI) LEAVES

Authors :

Kyatham Hemanth, S.K. Godasu, Sravanthi Kotha, Supraja Konakanti, Narni Jaya Prakash

Abstract :

Based on trial-and-error experience and folk remedies continue to draw wide attention for their role in the treatment of mild and chronic diseases. We focus on plant research has increased all over the world and a large body of evidence has been accumulated to highlight the immense potential of medicinal plants used in various traditional systems of medicine. Centella asiatica is an important medicinal herb that is widely used in the orient and is becoming popular in India. Triterpenoids, Saponins, the primary constituents of Centella asiatica are mainly believed to be responsible for its wide therapeutic actions. The herb is recommended for the treatment of various diseases like leprosy, lupus, varicose ulcers, eczema, psoriasis, diarrhea, fever, amenorrhea and Alzheimer’s. The present work employs the standardization and microscopical evaluation of leaf drug to authenticate by methodological parameters ranges of Stomatal number, Vein islet number and Stomatal index of the crud drug. Respective practical and morphological parameters of the drug of Centella asiatica . Various microsopical parameters to evaluate the characteristics are reported, which is imperative for the safe utilization of the herb, are discussed.
Key Words: Centella asiatica, description, stomata, safe utilization, authentication.

Cite This Article:

Please cite this article in press Kyatham Hemanth et al, Standardization and Microscopical Evaluation Studies of Centella Asiatica linn. (Brahmi) Leaves., Indo Am. J. P. Sci, 2022; 09(8).

,

Number of Downloads : 10

References:

1. Bandyopadhyay D, Biswas K, Bhattacharyya M, Reiter RJ, Banerjee RK. Gastric toxicity and mucosal ulceration induced by oxygen-derived reactive species, protection by melatonin. Curr Mol Med 2001;1:501-513.
2. Leslie W. Peptic Ulcer: A Reappraisal of its peptic aetiology. Ann Roy Coll Surg Engl 1972;50:146-163.
3. Bandyopadhyay D, Biswas K, Bhattacharyya M, Reiter RJ, Banerjee RK. Gastric toxicity and mucosal ulceration induced by oxygen-derived reactive species, protection by melatonin. Curr Mol Med 2001;1:501-513.
4. Leslie W. Peptic Ulcer: A Reappraisal of its peptic aetiology. Ann Roy Coll Surg Engl 1972;50:146-163
5. Jamil SS, Nizami Q, Salam M. Centella asiatica (Linn.) Urban: a review. Natural Product Radiance. 2007;6(2):158–170. [Google Scholar]
6. Meulenbeld GJ, Wujastyk D. Studies on Indian Medical History. New Delhi, India: Motilal Banarsidas; 2001. [Google Scholar]
7. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants (Including the Supplement) New Delhi, India: Council of Scientific and Industrial Research; 1986. [Google Scholar]
8. Howes MJR, Houghton P. Plants used in Chinese and Indian traditional medicine for improvement of memory and cognitive function. Pharmacology Biochemistry and Behavior. 2003;75(3):513–527. [PubMed] [Google Scholar]
9. Tenni R, Zanaboni G, De Agostini MP, Rossi A, Bendotti C, Cetta G. Effect of the triterpenoid fraction of Centella asiatica on macromolecules of the connective matrix in human skin fibroblast cultures. Italian Journal of Biochemistry. 1988;37(2):69–77. [PubMed] [Google Scholar]
10. Suguna L, Sivakumar P, Chandrakasan G. Effects of Centella asiatica extract on dermal wound healing in rats. Indian Journal of Experimental Biology. 1996;34(12):1208–1211. [PubMed] [Google Scholar]
11. Shetty BS, Udupa SL, Udupa AL. Biochemical analysis of granulation tissue in steroid and Centella asiatica (Linn) treated rats. Pharmacologyonline. 2008;2:624–632. [Google Scholar]
12. Somchit MN, Sulaiman MR, Zuraini A, et al. Antinociceptive and antiinflammatory effects of Centella asiatica . Indian Journal of Pharmacology. 2004;36(6):377–380. [Google Scholar]
13. George M, Joseph L, Ramaswamy Anti-allergic, anti-pruritic, and anti-inflammatory activities of Centella asiatica extracts. African Journal of Traditional, Complementary and Alternative Medicines. 2009;6(4):554–559. [PMC free article] [PubMed] [Google Scholar]
14. Sampson JH, Raman A, Karlsen G, Navsaria H, Leigh I. In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins. Phytomedicine. 2001;8(3):230–235. [PubMed] [Google Scholar]
15. Cheng CL, Koo MWL. Effects of Centella asiatica on ethanol induced gastric mucosal lesions in rats. Life Sciences. 2000;67(21):2647–2653. [PubMed] [Google Scholar]
16. Cheng CL, Guo JS, Luk J, Koo MWL. The healing effects of Centella extract and asiaticoside on acetic acid induced gastric ulcers in rats. Life Sciences. 2004;74(18):2237–2249. [PubMed] [Google Scholar]
17. Pingale SS. Evaluation of effect of Centella asiatica on CCL4 induced rat liver damage. Pharmacologyonline. 2008;3:537–543. [Google Scholar]
18. Sudha S, Kumaresan S, Amit A, David J, Venkataraman BV. Anti-convulsant activity of different extracts of Centella asiatica and Bacopa monnieri in animals. Journal of Natural Remedies. 2002;2(1):33–41. [Google Scholar]
19. Wijeweera P, Arnason JT, Koszycki D, Merali Z. Evaluation of anxiolytic properties of Gotukola—(Centella asiatica) extracts and asiaticoside in rat behavioral models. Phytomedicine. 2006;13(9-10):668–676. [PubMed] [Google Scholar]
20. Wang XS, Dong Q, Zuo JP, Fang JN. Structure and potential immunological activity of a pectin from Centella asiatica (L.) Urban. Carbohydrate Research. 2003;338(22):2393–2402. [PubMed] [Google Scholar]
21. Gnanapragasam A, Kumar Ebenezar K, Sathish V, Govindaraju P, Devaki T. Protective effect of Centella asiatica on antioxidant tissue defense system against adriamycin induced cardiomyopathy in rats. Life Sciences. 2004;76(5):585–597. [PubMed] [Google Scholar]
22. Raghavendra M, Maiti R, Kumar S, Trigunayat A, Mitra S, Acharya S. Role of Centella asiatica on cerebral post-ischemic reperfusion and long-term hypoperfusion in rats. International Journal of Green Pharmacy. 2009;3(2):88–96. [Google Scholar]
23. Venu Gopal Rao ML, Mastan SA. Antidiabetic effects of methanolic extract of Centella asiatica (Linn.) on induced hyperglycemic rats. Biosciences Biotechnology Research Asia. 2007;4(2):721–724. [Google Scholar]
24. Lee YS, Jin DQ, Kwon EJ, et al. Asiatic acid, a triterpene, induces apoptosis through intracellular Ca2+ release and enhanced expression of p53 in HepG2 human hepatoma cells. Cancer Letters. 2002;186(1):83–91. [PubMed] [Google Scholar]
25. Bunpo P, Kataoka K, Arimochi H, et al. Inhibitory effects of Centella asiatica on azoxymethane-induced aberrant crypt focus formation and carcinogenesis in the intestines of F344 rats. Food and Chemical Toxicology. 2004;42(12):1987–1997. [PubMed] [Google Scholar]
26. Yoosook C, Bunyapraphatsara N, Boonyakiat Y, Kantasuk C. Anti-herpes simplex virus activities of crude water extracts of Thai medicinal plants. Phytomedicine. 2000;6(6):411–419. [PubMed] [Google Scholar]
27. Zaidan MR, Noor Rain A, Badrul AR, Adlin A, Norazah A, Zakiah I. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Tropical Biomedicine. 2005;22(2):165–170. [PubMed] [Google Scholar]
28. Senthilkumar N, Varma P, Gurusubramanian G. Larvicidal and adulticidal activities of some medicinal plants against the Malarial Vector, Anopheles stephensi (Liston) Parasitology Research. 2009;104(2):237–244. [PubMed] [Google Scholar]
29. Naz E, Ahmad M. Evaluation of five indigenous medicinal plants of Sindh, Pakistan for their antifungal potential. Pakistan Journal of Scientific and Industrial Research. 2009;52(6):328–333. [Google Scholar]
30. Hamid AA, Shah Z, Muse R, Mohamed S. Characterisation of antioxidative activities of various extracts of Centella asiatica (L) Urban. Food Chemistry. 2002;77(4):465–469. [Google Scholar]
31. Jayashree G, Kurup Muraleedhara G, Sudarslal S, Jacob VB. Anti-oxidant activity of Centella asiatica on lymphoma-bearing mice. Fitoterapia. 2003;74(5):431–434. [PubMed] [Google Scholar]
32. Bajpai M, Pande A, Tewari SK, Prakash D. Phenolic contents and antioxidant activity of some food and medicinal plants. International Journal of Food Sciences and Nutrition. 2005;56(4):287–291. [PubMed] [Google Scholar]
33. Chaudhuri S, Ghosh S, Chakraborty T. Use of a common Indian herb “Mandukaparni” in the treatment of leprosy. (Preliminary report) Journal of the Indian Medical Association. 1978;70(8):177–180. [PubMed] [Google Scholar]
34. Pointel JP, Boccalon H, Cloarec M. Titrated extract of Centella asiatica (TECA) in the treatment of venous insufficiency of the lower limbs. Angiology. 1987;38(1):46–50. [PubMed] [Google Scholar]
35. Cesarone MR, Belcaro G, Rulo A, et al. Microcirculatory effects of total triterpenic fraction of Centella asiatica in chronic venous hypertension: measurement by laser Doppler, TcPo2-co2, and leg volumetry. Angiology. 2001;52(10):S45–S48. [PubMed] [Google Scholar]