Volume : 09, Issue : 12, December – 2022
Title:
29.PHYTOCHEMICALS & ANTI HYPERLIPIDAEMIC ACTIVITY OF METHANOLIC EXTRACT OF TEPHROSIA PURPUREAON RATS
Authors :
Ankit Gour*, Sonpal Singh, Jagdish Chandra Rathi
Abstract :
Natural products, particularly of plant origin, are the main quarry for discovering promising lead candidates and play an imperative role in the upcoming drug development programs. Ease of availability, low cost, and least side effects make plant-based preparations the main key player of all available therapies. Tephrosia purpurea is a wild herb belongs to the family fabaceae and commonly known as sharpunkha. This plant has also been used for the treatment of several gastrointestinal disorders and has ability to cure disorder related to bowel, kidney liver spleen. Its dried parts can be used effectively for the treatment of boils, bleeding piles, bronchitis etc. It also has diuretic property. Its roots decoction is useful in enlargement and damage of liver. The aim of this study is to examine anti-hyperlipidaemic effect of Tephrosia purpurea. The leaves of plant were collected and subjected to extraction by methanol as solvent. Further qualitative & quantitative estimation of phytochemical and antioxidant activity was also checked. The anti -hyperlipidaemic activity was analysed in rats by examining various biochemical parameters. The results showed that the yields were found to be 7.28% w/w of Tephrosia purpurea of petroleum ether extract, 12.54 % w/w of methanolic extract. The phytochemical screening revealed presence of alkaloid, flavonoid glycosides, tannins, steroid, phenol. Total phenol & flavonoid content was found to be 0.865 mg/100mg and 0.947 mg/100mg of dried extract respectively. The IC 50 value for Methanolic extract of Tephrosia purpurea was found to be 53.37 as compared to ascorbic acid with 17.68 IC 50value. All the treatment groups produced a significant decrease in serum TC, TG, HDL-C, and VLDL-C levels. In addition to the above, the serum LDL-C levels were significantly decreased by the Tephrosia purpurea extracts. The Tephrosia purpurea leaves extract significantly increased fecal cholesterol excretion on dose dependant manner. Thus, from the results it can be concluded that the methanolic extract of Tephrosia purpurea exhibit potent anti hyperlipedemic activity.
Keywords: Tephrosia purpurea, Anti -Hyperlipidaemic, Medicinal plants, TG, HDL, Cholesterol
Cite This Article:
Please cite this article in press Ankit Gour et al, Phytochemicals & Anti Hyperlipidaemic Activity Of Methanolic Extract Of Tephrosia Purpureaon Rats.., Indo Am. J. P. Sci, 2022; 09(12).
Number of Downloads : 10
References:
2. Asija R, Singh CH, Hemlata A. A comprehensive review on Antihyperlipidemic activity of various medicinal plants. Int J Curr Pharm Rev Res. 2016;7(6):407-15.
3. Koriem KM. Antihyperlipidemic activity of the medicinal plants among Kadazan and Dusun communities in Sabah, Malaysia: a review. Asian Pacific Journal of Tropical Biomedicine. 2014 Oct 1;4(10):768-79.
4. Rao AS, Yadav SS, Singh P, Nandal A, Singh N, Ganaie SA, Yadav N, Kumar R, Bhandoria MS, Bansal P. A comprehensive review on ethnomedicine, phytochemistry, pharmacology, and toxicity of Tephrosia purpurea (L.) Pers. Phytotherapy Research. 2020 Aug;34(8):1902-25.
5. Akanksha B, Avijit M, Chakraborthy GS, Seema G. Phytopharmacological uses of Tephrosia Purpurea—A review. Pharmacophore. 2014;5(4):658-65.
6. Dalwadi PP, Patel JL, Patani PV. Tephrosia purpurea Linn (Sharpunkha, Wild Indigo): a review on phytochemistry and pharmacological studies. Indian Journal of Pharmaceutical and Biological Research. 2014 Jan 1;2(1):108.
7. Babu N, Singh A, Singh R. A review on therapeutic potential and phytochemistry of Tephrosia purpurea. Bulletin of Pure & Applied Sciences-Botany. 2017 Jul 1(2).
8. Kokate CK. Ed. Practical Pharmacognosy, 4th Edn., Vallabh Prakashan: 1994; 112:120.
9. Harborne JB. Phytochemical methods A guide to modern techniques of plant analysis. Third ed. Chapman and Hall. 1998; 204-8.
10. Mishra AG, Singh R, Meha P, Parkhe G. Determination of total phenolic, flavonoid content, antioxidant and antimicrobial activity of Gloriosa superb seed extract. Asian J Pharm Educ Res. 2017;6(2):12-7.
11. Parkhe G, Jain P. Study of antioxidant potential of hydroalcoholic extract of Anethum graveolens. Career. Int J Sci Technol. 2018;1(2):39-45.
12. Blank B, Pfeiffer FR, Greenberg CM, Kerwin JF (1963): Thyromimetics.II. The synthesis and hypocholesterolemic activity of some betadimethylaminoethyl esters of iodinated thyroalkanoic acids. J Med Chem 6: 560–563.
13. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry. 1972 Jun 1;18(6):499-502.
14. Zlatkis A, Zak B, Boyle AJ. A new method for the direct determination of serum cholesterol. The Journal of laboratory and clinical medicine. 1953 Mar 1;41(3):486-92.
15. Foster LB, Dunn RT. Stable reagents for determination of serum triglycerides by a colorimetric Hantzsch condensation method. Clinical chemistry. 1973 Mar 1;19(3):338-40.