Volume : 09, Issue : 12, December – 2022
Title:
91.SIMULTANEOUS ESTIMATION OF PRAZOSIN AND POLYTHIAZIDE IN BULK AND FIXED DOSE COMBINATION (TABLETS) BY RP-HPLC
Authors :
M. Suma Pallavi, P.Prapulla
Abstract :
A simple, Accurate, precise method was developed for the simultaneous estimation of the Prazosin and Polythiazide in Tablet dosage form. Chromatogram was run through Std Discovery C18 150 x 4.6 mm, 5µ. Mobile phase containing Buffer 0.1%OPA: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 270.0 nm. Retention time of Prazosin and Polythiazide were found to be 2.316 min and 3.176. %RSD of the Prazosin and Polythiazide were found to be 0.5 and 0.7 respectively. %Recovery was obtained as 100.49% and 100.40% for Prazosin and Polythiazide respectively. LOD, LOQ values obtained from regression equations of Prazosin and Polythiazide were 0.079, 0.240 and 0.03, 0.08 respectively. Regression equation of Prazosin is y = 50050.x + 7773 and of Polythiazide was y = 95434.x + 6175. Retention times were decreased and run time was decreased, so the method developed was simple and times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
Cite This Article:
Please cite this article in press M. Suma Pallavi et al, Simultaneous Estimation Of Prazosin And Polythiazide In Bulk And Fixed Dose Combination (Tablets) By RP-HPLC.., Indo Am. J. P. Sci, 2022; 09(12).
Number of Downloads : 10
References:
:
1. Stanaszek WF, Kellerman D, Brogden RN, Romankiewicz JA: Prazosin update. A review of its pharmacological properties and therapeutic use in hypertension and congestive heart failure. Drugs. 1983 Apr;25(4):339-84.
2. Kung S, Espinel Z, Lapid MI: Treatment of nightmares with prazosin: a systematic review. Mayo Clin Proc. 2012 Sep;87(9):890-900.
3. William JB. High Performance Liquid Chromatographic Determination of Diuretic-Antihypertensive Combination Products. I. Prazosin and Polythiazide. Journal of Liquid Chromatography. 1986; 9(5):1033-1049.
4. Shrivastava A, Gupta VB. Stability-Indicating RP-HPLC Method for the Simultaneous Determination of Prazosin, Terazosin, and Doxazosin in Pharmaceutical Formulations. Sci Pharm. 2012; 80(3): 619–631.
5. Tenjarla SN, Tseggai A. High-performance liquid chromatographic assay of prazosin for transdermal screening studies. Journal of Clinical Pharmacy and Therapeutics.1992; 17(1): 37–42.
6. Shrivastava A, Gupta VB. Simultaneous determination of two alpha¬one adrenoreceptor blockers Terazosin and Prazosin using Tamsulosin as internal standard. International Journal of Pharmacy and Pharmaceutical Sciences. 2012; 4(3):752-756.
7. Moskalyk RE, Locock RA, Chatten LG, Veltman AM, Monique FB. Determination of polythiazide in pharmaceutical dosage forms by high‐pressure liquid chromatography. 1975; 64(8):1406-1408.
8. Bakshi M, Ojha T, Singh S. Validated specific HPLC methods for determination of prazosin, terazosin and doxazosin in the presence of degradation products formed under ICH-recommended stress conditions. J Pharm Biomed Anal. 2004; 34(1):19-26.
9. Sultana N, Saeed MA, Shah SN. Liquid Chromatographic Analysis of Prazosin in API, Dosage Form and Serum: Application to Drug-Metal Interaction Studies. J Chromatograph Separat Techniq. 2013; 4(7):1-5.
10. Bakshi M, Ojha T, Singh S. Validated specific HPLC methods for determination of prazosin, terazosin and doxazosin in the presence of degradation products formed under ICH-recommended stress conditions. Journal of Pharmaceutical and Biomedical Analysis. 2004; 34(1):19-26.
11. Ramolia C, Dedania Z, Dedania R, Sheth NR, Vidya sagar G, Patel B, Bhatt KK. Simultaneous Estimation of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride in Pharmaceutical Dosage Forms by RP-HPLC Method. Asian J. Research Chem.2010; 3(1): 83-86.
12. Patil S, Ramesh B, Hareesh AR, Patil K, Dhokane A. Development and Validation of RP-HPLC Method for the Estimation of Sitagliptin Phosphate in Tablet Dosage Form. Asian J. Research Chem. 2010; 3(3): 653-655.
13. Maste MM, Parate AN, Bhat AR. Validation and Application of a High-Performance Liquid Chromatography Method for Estimation of Sitagliptin Phosphate from Bulk Drug and Pharmaceutical Formulation. Asian J. Research Chem.2011; 4(9):1466-1468.
14. Dighe NS, Shinde GS, Vikhe JJ. Simultaneous Estimation, Validation and Force Degradation Study of Metformin Hydrochloride and Empagliflozin by RP-HPLC Method. Research J. Science and Tech. 2019; 11(2):135-147.
15. Baokar SB, Mulgund SV, Ranpise NS. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Vildagliptin and Metformin. Research J. Pharma. Dosage Forms and Tech. 2013; 5(2): 95-98.
16. Kalyankar TM, Kakde RB. Reversed-Phase Liquid Chromatographic Method for Simultaneous Determination of Metformin and Repaglinide in Pharmaceutical Preparations. Research J. Pharma. Dosage Forms and Tech. 2011; 3(5): 233-237.
17. More SS, Sonawane SS, Chhajed SS, Kshirsagar SJ. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Tablets. Asian J. Pharm. Tech. 2018; 8(3):145-148.
18. Panigrahy UP, Reddy ASK. A novel validated RP-HPLC-DAD method for the estimation of Eluxadoline in bulk and pharmaceutical dosage form. Research J. Pharm. and Tech. 2015; 8(11):1469-1476.
19. Lobhe GA, Shah A, Singhvi I. Development and Validation of a Stability-Indicating RP-HPLC Method for the Determination of Sitagliptin Phosphate and Simvastatin in the Presence of their Degradation Products in Bulk and Binary Mixture. Asian J. Res. Pharm. Sci. 2016; 6(3): 191-197.
20. Panigrahy UP, Panda SP, Sudhakar M. Colorimetric Analysis of Raltegravir in Pharmaceutical Formulation and Human Biological Fluids through Oxidative Coupling Reaction using 3-Methyl-2-Benzothiazolinone Hydrazone Reagent. Research J. Pharm. and Tech. 2018; 11(5):1788-1793.
21. Mahmure U, Sungur S. A Spectrophotometric Method for the Determination of Prazosin Hydrochloride in Tablets. Turk J Chem. 2002; 26: 691 – 696.
22. Nikam P, Baokar S, Undare S, Patil RN. Spectrophotometric Method Development and Validation of Prazosin. Research J. Science and Tech.2016; 8(1): 1-4.
23. Biljana P, Miroslava I, Gordana T, Bogdan B. Simultaneous determination of polythiazide and prazosin in tablets by second-order derivative UV spectroscopy. International Journal of Pharmaceutics. 1991; 70 (1–2): 187-190.
24. Jie L, Bi-kui Z, Ben-mei C, Huan-de L. HPLC-MS determination of prazosin in the human plasma and its bioequivalence. Chinese Journal of Pharmaceutical Analysis. 2006; 26(5): 621-624.
25. Ning MA, Wen-ying L, Huan-de L, Ben-mei C, Bi-kui Z. HPLC-MS/ESI determination of terazosin hydrochloride, prazosin hydrochloride and doxazosin mesyllate in human plasma. Chinese Journal of Pharmaceutical Analysis. 2007; 27(10):1548-1552.
26. Shabir GA. Validation of high-performance liquid chromatography methods for pharmaceutical analysis. Understanding the differences and similarities between validation requirements of the US food and drug administration, the US Pharmacopeia and the International Conference on Harmonization. J Chromatogr A. 2003; 987: 57-66.