Volume : 09, Issue : 12, December – 2022
Title:
92.SIMULTANEOUS ESTIMATION OF SERTRALINE AND ALPRAZOLAM IN ITS BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD
Authors :
B.Sanghavi, P.Prapulla
Abstract :
A simple, accurate, rapid and precise isocratic reversed-phase high- performance liquid chromatographic method has been developed and validated for simultaneous determination of Sertraline and Alprazolam in tablets. The chromatographic separation was carried out on an cosmosil packed column 5c-18 ms II (250×4.6 i.d ) with a mixture of acetonitrile: methanol: phosphate buffer pH 3 adjusted with orthophosphoric acid (20:50:30, v/v) as mobile phase; at a flow rate of1.0 ml/min. UV detection was performed at 239 nm. The retention times were 4.915 and 8.056 min. for Sertraline and Alprazolam respectively. Calibration plots were linear (r2>0.998) over the concentration range 10-60 μg/ml for sertraline and 10-60 μg/ml Alprazolam. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. The proposed method was successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of Sertraline and Alprazolam in bulk drug and tablet dosage form.
Keywords: Sertraline, Alprazolam, RP‐HPLC, Simultaneous estimation.
Cite This Article:
Please cite this article in press B.Sanghavi et al, Simultaneous Estimation Of Sertraline And Alprazolam In Its Bulk And Pharmaceutical Dosage Form By RP-HPLC Method.., Indo Am. J. P. Sci, 2022; 09(12).
Number of Downloads : 10
References:
:
1. FDA Approved Drug Products: Zoloft (sertraline hydrochloride) for oral use
2. Feng R, Wang P, Gao C, Yang J, Chen Z, Yang Y, Jiao J, Li M, Fu B, Li L, Zhang Z, Wang S: Effect of sertraline in the treatment and prevention of poststroke depression: A meta-analysis. Medicine (Baltimore). 2018 Dec;97(49):e13453. doi: 10.1097/MD.0000000000013453. (PubMed ID 30544429)
3. Rang, H. P. and Dale, M. M. (2012). Rang and Dale’s Pharmacology (7th ed.). Edinburgh: Elsevier/Churchill Livingstone. (ISBN 978-0-7020-3471-8)
4. Pittenger C, Bloch MH, Williams K: Glutamate abnormalities in obsessive compulsive disorder: neurobiology, pathophysiology, and treatment. Pharmacol Ther. 2011 Dec;132(3):314-32. doi: 10.1016/j.pharmthera.2011.09.006. Epub 2011 Sep 22. (PubMed ID 21963369)
5. FDA Approved Drug Products: XANAX (alprazolam) tablets
6. Sanjay B, Manank Bhavsar, Indrajeet Singhvi, Anil Chandewar. Analytical method development and validation for the estimation of Alprazolam and Sertraline Hydrochloride by HPLC. Journal of Pharma Research. 11(1); 2014: 6-16.
7. Ramya S, Gowri Sankar D, Chaitanya KK, Gowri P. Simultaneous Determination of Alprazolam and Sertraline by RP-HPLC.Indian Journal of Pharmaceutical Sciences. 58(7); 2005: 352-360.
8. Lijuan He, Fang Feng, Jie Wu. Determination of Sertraline in Human plasma by HPLC-Electro sprays Ionisation mass spectrometry and method validation. J Chromatogr Sci. 2(3):2005: 43.
9. Venkateshwarlu K,Venisetty RK,Yellu NR, Keshetty S, Pai MG. Develpoment of HPTLC-UV absorption densitometry method for the analysis of Alprazolam and Sertraline in combination and its application in the evaluation of marketed preparations. J Chromatogr Sci. 45(2); 2007:537-539.
10. Amir Farshchi, Golbarg Ghiasi, Gholamreza, Bahrami.High Performance Liquid Chromatography determination of Sertraline in Human plasma. Iranian Journal of Pharmaceutical Sciences. 5(3); 2009:171-178.