Volume : 09, Issue : 01, January – 2022
06.ISOLATION AND CHARACTERIZATION OF QUERCETIN IN MATHUKA NERIIFOLIA (MOON) H.J. LAM. ETHANOL EXTRACT
Dr. Minu. B. Pattasseril*, Dr. Sujith Abraham
Herbal medicine is making a dramatic comeback as the side effect of synthetic medicine are daunting and the therapeutic approach is drifting towards substituent medicine. Quercetin is a plant pigment flavonoid. It’s found in many plants and foods, such as red wine, onions, green tea, apples, and berries. Quercetin has antioxidant and anti-inflammatory effects that might help reduce swelling, kill cancer cells, control blood sugar, and help prevent heart disease. Madhuca neriifolia (Moon) H.J. Lam. is an endangered plant species occurring in the Southern, Western Ghats of India. Flowers are used in the treatment of kidney complaints. Fruits are recommended in cases of rheumatism, biliousness, consumption, asthma and worm trouble. Oil from seeds is used to treat rheumatism and for improved growth of hair. Fractnation of ethanol extract of Mathuka Neriifolia (Moon) H.J. Lam. by column chromatography was done. During the column elution process, the fractions 260 – 300 has a single banding pattern which was confirmed by TLC study. Phyochemical screening showed the presence of Flavonoids , Phenolic and Tannins. Physical and chemical tests of isolated compound was conducted. By using IR, LC-MS, 1H-NMR and 13C-NMR spectra of the isolated compound was confirmed as Quercetin.
Keywords: Quercetin, Mathuka Neriifolia (Moon) H.J. Lam., Phytochemical screening, flavonoid.
Cite This Article:
Please cite this article in press Minu . B. Pattasseril et al, Isolation and characterization of quercetin in mathuka neriifolia (moon) H.J. Lam. Ethanol extract., Indo Am. J. P. Sci, 2022; 09(01).
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1. A. J. Stewart, S. Bozonnet, W. Mullen, G. I. Jenkins, M. E. Lean, and A. Crozier, “Occurrence of flavonols in tomatoes and tomato-based products,” Journal of Agricultural and Food Chemistry, vol. 48, no. 7, pp. 2663–2669, 2000.
2. A. K. Pandey, “Anti-staphylococcal activity of a pan-tropical aggressive and obnoxious weedParihenium histerophorus: an in vitro study,” National Academy Science Letters, vol. 30, no. 11-12, pp. 383–386, 2007.
3. Amić, D.; Davidović-Amić, D.; Bešlo, D.; Trinajstić, N. Structure-radical scavenging activity relationships of flavonoids. Croat. Chem. Acta 2003, 76, 55–61.
4. Annil Mahajan, Vishal R Tandon J. 2004 ;Antioxidants and rheumatoid arthritis. Indian Rheumatol Assoc , 12 : 139 – 142.
5. Bohm, B. Introduction of Flavonoids; Harwood Academic Publishers: Singapore, 1998.
6. Buer, C.S.; Imin, N.; Djordjevic, M.A. Flavonoids: New roles for old molecules. J. Integr. Plant Biol. 2010, 52, 98–111.
7. C. A. Rice-Evans, N. J. Miller, P. G. Bolwell, P. M. Broamley, and J. B. Pridham, “The relative antioxidant activities of plant-derived polyphenolic flavonoids,” Free Radical Research, vol. 22, no. 4, pp. 375–383, 1995.
8. Cotelle, N.; Bernier, J.L.; Catteau, J.P.; Pommery, J.; Wallet, J.C.; Gaydou, E.M. Antioxidant properties of hydroxy-flavones. Free Radic. Biol. Med. 1996, 20, 35–43.
9. de la Rosa L.A., Alvarez-Parrilla E., Gonzàlez-Aguilar G.A. Fruit and vegetable phytochemicals: chemistry, nutritional value, and stability. 1th Edition. Wiley J. & Sons, Inc., Publication, 2010
10. E. H. Kelly, R. T. Anthony, and J. B. Dennis, “Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships,” Journal of Nutritional Biochemistry, vol. 13, no. 10, pp. 572–584, 2002.
11. E. J. Middleton, “Effect of plant flavonoids on immune and inflammatory cell function,” Advances in Experimental Medicine and Biology, vol. 439, pp. 175–182, 1998.
12. Enos Tangke Arung, Britanto Dani Wicaksono, Yohana Ayupriyanti Handoko, Irawan Wijaya Kusuma, Dina Yulia1 and Ferry Sandra. , 2009; Anti-Cancer Properties of Diethylether Extract of Wood from Sukun (Artocarpus altilis) in Human Breast Cancer (T47D) Cells .Tropical Journal of Pharmaceutical Research, 8 (4): 317-32.
13. F. Du, F. Zhang, F. Chen et al., “Advances in microbial heterologous production of flavonoids,” African Journal of Microbiology Research, vol. 5, no. 18, pp. 2566–2574, 2011.
14. Forkmann, G.; Heller, W. Biosynthesis of flavonoids. In Comprehensive Natural Products Chemistry; Elsevier: Amsterdam, The Netherlands, 1999; pp. 713–748.
15. G. Agati, E. Azzarello, S. Pollastri, and M. Tattini, “Flavonoids as antioxidants in plants: location and functional significance,” Plant Science, vol. 196, pp. 67–76, 2012.
16. Han X., Shen T. and Lou H. Dietary polyphenols and their biological significance. Int J Mol Sci 2007;9:950-988. doi:10.3390/i8090950
17. Hetal Amin, Rohit Sharma, Mahesh Vyas, PK Prajapati, Kartar Dhiman,2014;Validation of the ayurvedic therapeutic claims through contemporary studies. International jourmal of green pharmacy, 8(4): 193.
18. Irena Matlawska and Maria Sikorska. 2005;Flavonoids from Abutilon Theophrasti flowers.Acta poloniae Pharmaceutical and drug research, vol. 62 no. 2 pp. 135-39.
19. J B Harborne. 1998;Phytochemical methods ,A guide to modern techniques of plant analysis.Third edition. 40-44,73-79.
20. K. Reinli and G. Block, “Phytoestrogen content of foods: a compendium of literature values,” Nutrition and Cancer, vol. 26, no. 2, pp. 123–148, 1996.
21. Khandelwal KR. 2006; Practical Pharmacognosy Techniques And Experiments,15thED,Nirali Prakashan,India.
22. Kokate CC, Purohit AP and Gokhale SB. 2001; Text Book of Pharmacognosy, 7th ED, Nirali Prakashan, India.
23. Krishnaiah D, Sarbatly R, Nithyanandam RR., 2011;A review of the antioxidant potential of medicinal plant species. Food Bioprod Process, 89:217–233
24. Lorenzo V., Greco and Marco N. Bruno. 2008;Food science and technology:new research. Nova science publishers,. New York. Page:24-28.
25. M. F. Mahomoodally, A. Gurib-Fakim, and A. H. Subratty, “Antimicrobial activities and phytochemical profiles of endemic medicinal plants of Mauritius,” Pharmaceutical Biology, vol. 43, no. 3, pp. 237–242, 2005.
26. M. G. L. Hertog, P. C. H. Hollman, and M. B. Katan, “Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands,” Journal of Agricultural and Food Chemistry, vol. 40, no. 12, pp. 2379–2383, 1992.
27. M. L. Lázaro, “Distribution and biological activities of the flavonoid luteolin,” Mini-Reviews in Medicinal Chemistry, vol. 9, no. 1, pp. 31–59, 2009.
28. M. Leopoldini, N. Russo, S. Chiodo, and M. Toscano, “Iron chelation by the powerful antioxidant flavonoid quercetin,” Journal of Agricultural and Food Chemistry, vol. 54, no. 17, pp. 6343–6351, 2006.
29. M. Lopez, F. Martinez, C. Del Valle, C. Orte, and M. Miro, “Analysis of phenolic constituents of biological interest in red wines by highperformance liquid chromatography,” Journal of Chromatography A, vol. 922, no. 1-2, pp. 359–363, 2001.
30. Manach C., Scalbert A., Morand C., Rémésy C., and Jime´nez L. Polyphenols: food sources and bioavailability. Am J Clin Nutr 2004;79(5):727-47
31. Maria Kratchanova, Petko Denev, Milan Ciz, Antonin Lojek and Atanas Mihailov,2010 ;Evaluation of antioxidant activity of medicinal plants containing polyphenol compounds. Comparison of two extraction systems.ACTA Biochimica polonica. Vol. 57, No. 2/ 229–234.
32. Middeltone, H. 1956 ;Systematic Qualitative Analysis. Edward Arnnold Publishers Ltd., London.
33. Murphy Karen J, Chronopoulos Andriana K, Singh I, el al. Dietary flavanols and procyanidin oligomers from cocoa (Theobroma cacao) inhibit platelet function. American Journal of Clinical Nutrition 2003;77(6):1466-73.
34. N. C. Cook and S. Samman, “Review: flavonoids-chemistry, metabolism, cardioprotective effects and dietary sources,” Journal of Nutritional Biochemistry, vol. 7, no. 2, pp. 66–76, 1996.
35. Peach T , Trancey MV. Modern Methods in Plant Analysis. Springer Verlog, Berlin; 1955
36. Prasanna Kumar C N, Shivaprasad D, Somashekar R K, Nagaraja B C. , 2013; Reproductive Phenology and Pollination Biology of Madhuca neriifolia in wet evergreen forest of Western Ghats, South India. International Journal of Advanced Research , 1( 9), 296-306
37. R. A. Dixon, P. M. Dey, and C. J. Lamb, “Phytoalexins: enzymology and molecular biology,” Advances in Enzymology and Related Areas of Molecular Biology, vol. 55, pp. 1–136, 1983.
38. R. L. Rousseff, S. F. Martin, and C. O. Youtsey, “Quantitative survey of narirutin, naringin, hesperidin, and neohesperidin in citrus,” Journal of Agricultural and Food Chemistry, vol. 35, no. 6, pp. 1027–1030, 1987.
39. R.B.Kshatriyaa, Dr.G.M.Nazeruddin , bioactive bioactive bioactive flavonoids flavonoids flavonoids of therapeutic importance, 7 th International Symposium on Feedstock Recycling of Polymeric Materials (7th ISFR 2013) New Delhi, India, 23-26 October 2013
40. Rice-Evans, C.A.; Miller, N.J.; Paganga, G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 1996, 20, 933–956.
41. Rosenthaler L. 2011; Chemical investigations of plants. G. Bell and Sons, London; 1930.
42. S. Kreft, M. Knapp, and I. Kreft, “Extraction of rutin from buckwheat (Fagopyrum esculentum Moench) seeds and determination by capillary electrophoresis,” Journal of Agricultural and Food Chemistry, vol. 47, no. 11, pp. 4649–4652, 1999.
43. S. Kumar and A. K. Pandey, “Phenolic content, reducing power and membrane protective activities ofSolanum xanthocarpum root extracts,” Vegetos, vol. 26, pp. 301–307, 2013.
44. S. Kumar, A. Gupta, and A. K. Pandey, “Calotropis procera root extract has capability to combat free radical mediated damage,” ISRN Pharmacology, vol. 2013, Article ID 691372, 8 pages, 2013.
45. S. Kumar, A. Mishra, and A. K. Pandey, “Antioxidant mediated protective effect of Parthenium hysterophorus against oxidative damage using in vitro models,” BMC Complementary and Alternative Medicine, vol. 13, article 120, 2013.
46. Scholars Research Library. 2012; J. Nat. Prod. Plant Resour, 2 (4):512-516.
47. Seyoum, A.; Asres, K.; El-Fiky, F.K. Structure-radical scavenging activity relationships of flavonoids. Phytochemistry 2006, 67, 2058– 2070.
48. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients 2010;2:1231-1246. doi:10.3390/nu2121231
49. Y. Hara, S. J. Luo, R. L. Wickremasinghe, and T. Yamanishi, “Special issue on tea,” Food Reviews International, vol. 11, pp. 371–542, 1995.
50. Y. Miyake, K. Shimoi, S. Kumazawa, K. Yamamoto, N. Kinae, and T. Osawa, “Identification and antioxidant activity of flavonoid metabolites in plasma and urine of eriocitrin-treated rats,” Journal of Agricultural and Food Chemistry, vol. 48, no. 8, pp. 3217–3224, 2000.