Volume : 09, Issue : 07, July – 2022



Authors :

Gouri Jayachandran M

Abstract :

Vesicular drug delivery systems are promising agents with diverse applications in pharmaceuticals, cosmeceuticals and cosmetics. Bilosomes are bilayered vesicles of non-ionic surfactants and bile salts which are similar to niosomes. Bile salts are endogenous surfactants which act as a double-edged sword by increasing the aqueous solubility and permeability of active pharmaceutical ingredient. These vesicular structures increase the solubility of lipophilic drugs and increase the stability of the formulation in the gastrointestinal tract. Bilosomes are ultradeformable flexible structures which increases the stratum corneum permeability. Thus these have applications in both oral and transdermal drug delivery. These vesicles are utilised for topical drug delivery like ocular and intranasal drug delivery. In addition, bile salt integrated nanomedicines like probilosomes, surface engineered bilosomes and non-oral bilosomes have been surveyed. Tremendous research in the last decade has made bilosomes a potential carrier system. The extensive search has been presented related to the formulation and characterisation of bilosomes. Bilosomal systems have profound application in biological therapeutics and vaccine delivery. This review offers a comprehensive and informative data focusing on the great potential of bile acid and their salts for therapeutic application. In conclusion, bilosomes are superior to other conventional vesicular carriers (liposome and niosome) with regards to the stability, low toxicity and bioavailability.
Key words: Vesicular drug delivery system, bilosome, endogenous surfactants, aqueous solubility, permeability, ultradeformable, nanomedicines, biological therapeutics, vaccine delivery, bioavailability

Cite This Article:

Please cite this article in press Gouri Jayachandran M, Bilosomes: A Novel Vesicular Carrier for Drug Delivery – A Review., Indo Am. J. P. Sci, 2022; 09(7).,

Number of Downloads : 10



1. Varshney M, Mohan S. Nanotechnology” current status in pharmaceutical science. A, IJTA. 2012;6:14-24.
2. Chilkawar R, Nanjwade B, Nwaji M, Idris S, Mohamied A. Bilosomes based drug delivery system. J. Chem. Appl. 2015;2(5).
3. Shukla A, Khatri K, Gupta PN, Goyal AK, Mehta A, Vyas SP. Oral immunization against hepatitis B using bile salt stabilized vesicles (bilosomes). Journal of Pharmacy & Pharmaceutical Sciences. 2008 Mar 3;11(1):59-66.
4. Ahmed S, Kassem MA, Sayed S. Bilosomes as promising nanovesicular carriers for improved transdermal delivery: construction, in vitro optimization, ex vivo permeation and in vivo evaluation. International Journal of Nanomedicine. 2020;15:9783.
5. Rajput T, Chauhan MK. Bilosome: a bile salt based novel carrier system gaining interest in pharmaceutical research. Journal of Drug Delivery and Therapeutics. 2017 Sep 13;7(5):4-16.
6. Al-Mahallawi AM, Abdelbary AA, Aburahma MH. Investigating the potential of employing bilosomes as a novel vesicular carrier for transdermal delivery of tenoxicam. International journal of pharmaceutics. 2015 May 15;485(1-2):329-40.
7. Faustino C, Serafim C, Rijo P, Reis CP. Bile acids and bile acid derivatives: use in drug delivery systems and as therapeutic agents. Expert Opinion on Drug Delivery. 2016 Aug 2;13(8):1133-48.
8. Chacko IA, Ghate VM, Dsouza L, Lewis SA. Lipid vesicles: A versatile drug delivery platform for dermal and transdermal applications. Colloids and Surfaces B: Biointerfaces. 2020 Nov 1;195:111262.
9. Arafat M, Kirchhoefer C, Mikov MM, Sarfraz MK, Löbenberg R. Nanosized liposomes containing bile salt: A vesicular nanocarrier for enhancing oral bioavailability of BCS class III drug.
10. Mohsen AM, Salama A, Kassem AA. Development of acetazolamide loaded bilosomes for improved ocular delivery: Preparation, characterization and in vivo evaluation. Journal of Drug Delivery Science and Technology. 2020 Oct 1;59:101910.
11. Elnaggar YS. Multifaceted applications of bile salts in pharmacy: an emphasis on nanomedicine. International journal of nanomedicine. 2015;10:3955.
12. Chilkawar R, Nanjwade B, Nwaji M, Idris S, Mohamied A. Bilosomes based drug delivery system. J. Chem. Appl. 2015;2(5).
13. Palekar-Shanbhag P, Lande S, Chandra R, Rane D. Bilosomes: Superior Vesicular Carriers. Current Drug Therapy. 2020 Aug 1;15(4):312-20.
14. Zafar A, Alruwaili NK, Imam SS, Alotaibi NH, Alharbi KS, Afzal M, Ali R, Alshehri S, Alzarea SI, Elmowafy M, Alhakamy NA. Bioactive Apigenin loaded oral nano bilosomes: Formulation optimization to preclinical assessment. Saudi Pharmaceutical Journal. 2021 Mar 1;29(3):269-79.
15. Myneni GS, Radha GV, Soujanya GV. Novel Vesicular Drug Delivery Systems: A Review. Journal of Pharmaceutical Research. 2021;11(04).
16. Haritha P, Lakshmi PK. Probilosomes: A Novel bile salt containing nanocarrier for enhancing oral bioavailability. International Journal of Pharmaceutical Investigation. 2020 Mar 12;10(1):49-53.
17. Wilkhu JS, McNeil SE, Anderson DE, Perrie Y. Characterization and optimization of bilosomes for oral vaccine delivery. Journal of drug targeting. 2013 Apr 1;21(3):291-9.
18. Jain S, Harde H, Indulkar A, Agrawal AK. Improved stability and immunological potential of tetanus toxoid containing surface engineered bilosomes following oral administration. Nanomedicine: Nanotechnology, Biology and Medicine. 2014 Feb 1;10(2):431-40.
19. Ahmad J, Singhal M, Amin S, Rizwanullah M, Akhter S, Amjad Kamal M, Haider N, Midoux P, Pichon C. Bile salt stabilized vesicles (bilosomes): a novel nano-pharmaceutical design for oral delivery of proteins and peptides. Current pharmaceutical design. 2017 Mar 1;23(11):1575-88.
20. Aburahma MH. Bile salts-containing vesicles: promising pharmaceutical carriers for oral delivery of poorly water-soluble drugs and peptide/protein-based therapeutics or vaccines. Drug delivery. 2016 Jul 23;23(6):1847-67.
21. Niu M, Tan YN, Guan P, Hovgaard L, Lu Y, Qi J, Lian R, Li X, Wu W. Enhanced oral absorption of insulin-loaded liposomes containing bile salts: a mechanistic study. International journal of pharmaceutics. 2014 Jan 2;460(1-2):119-30.
22. El-Nabarawi MA, Shamma RN, Farouk F, Nasralla SM. Bilosomes as a novel carrier for the cutaneous delivery for dapsone as a potential treatment of acne: preparation, characterization and in vivo skin deposition assay. Journal of Liposome Research. 2020 Jan 2;30(1):1-1.
23. Salama A, El-Hashemy HA, Darwish AB. Formulation and optimization of lornoxicam-loaded bilosomes using 23 full factorial design for the management of osteoarthritis in rats: Modulation of MAPK/Erk1 signaling pathway. Journal of Drug Delivery Science and Technology. 2022 Mar 1;69:103175.
24. Abdelbary AA, Abd-Elsalam WH, Al-Mahallawi AM. Fabrication of novel ultradeformable bilosomes for enhanced ocular delivery of terconazole: in vitro characterization, ex vivo permeation and in vivo safety assessment. International journal of pharmaceutics. 2016 Nov 20;513(1-2):688-96.
25. D’Elia RV, Woods S, Butcher W, McGahon J, Khadke S, Perrie Y, Williamson ED, Roberts CW. Exploitation of the bilosome platform technology to formulate antibiotics and enhance efficacy of melioidosis treatments. Journal of controlled release. 2019 Mar 28;298:202-12.
26. Sharpe LA. Development and characterization of microencapsulated nanoparticle systems for oral vaccination by protein-antigens (Doctoral dissertation).
27. Khalil RM, Abdelbary A, Kocova El-Arini S, Basha M, El-Hashemy HA. Evaluation of bilosomes as nanocarriers for transdermal delivery of tizanidine hydrochloride: in vitro and ex vivo optimization. Journal of liposome research. 2019 Apr 3;29(2):171-82.
28. Saifi Z, Rizwanullah M, Mir SR, Amin S. Bilosomes nanocarriers for improved oral bioavailability of acyclovir: A complete characterization through in vitro, ex-vivo and in vivo assessment. Journal of Drug Delivery Science and Technology. 2020 Jun 1;57:101634.
29. Waglewska E, Pucek-Kaczmarek A, Bazylińska U. Self-assembled bilosomes with stimuli-responsive properties as bioinspired dual-tunable nanoplatform for pH/temperature-triggered release of hybrid cargo. Colloids and Surfaces B: Biointerfaces. 2022 Jul 1;215:112524.
30. Mosallam S, Sheta NM, Elshafeey AH, Abdelbary AA. Fabrication of highly deformable bilosomes for enhancing the topical delivery of terconazole: in vitro characterization, microbiological evaluation, and in vivo skin deposition study. AAPS PharmSciTech. 2021 Feb;22(2):1-2.
31. Palekar-Shanbhag P, Lande S, Chandra R, Rane D. Bilosomes: Superior Vesicular Carriers. Current Drug Therapy. 2020 Aug 1;15(4):312-20.
32. Elnaggar YS, Omran S, Hazzah HA, Abdallah OY. Anionic versus cationic bilosomes as oral nanocarriers for enhanced delivery of the hydrophilic drug risedronate. International Journal of Pharmaceutics. 2019 Jun 10;564:410-25.
33. Song KH, Chung SJ, Shim CK. Enhanced intestinal absorption of salmon calcitonin (sCT) from proliposomes containing bile salts. Journal of Controlled Release. 2005 Sep 2;106(3):298-308.
34. Albash R, Al-Mahallawi AM, Hassan M, Alaa-Eldin AA. Development and optimization of terpene-enriched vesicles (Terpesomes) for effective ocular delivery of fenticonazole nitrate: in vitro characterization and in vivo assessment. International Journal of Nanomedicine. 2021;16:609.
35. Chen Y, Jiang Z, Xu J, Zhang J, Sun R, Zhou J, Lu Y, Gong Z, Huang J, Shen X, Du Q. Improving the ameliorative effects of berberine and curcumin combination via dextran-coated bilosomes on non-alcohol fatty liver disease in mice. Journal of nanobiotechnology. 2021 Dec;19(1):1-8.
36. Albash R, El-Nabarawi MA, Refai H, Abdelbary AA. Tailoring of PEGylated bilosomes for promoting the transdermal delivery of olmesartan medoxomil: in-vitro characterization, ex-vivo permeation and in-vivo assessment. International Journal of Nanomedicine. 2019;14:6555.