Volume : 09, Issue : 06, June – 2022
Title:
36.METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF SULFAMETHOXAZOLE AND TRIMETHOPRIM BY RP-HPLC
Authors :
Syed Luqman Al Jeelani Quadri *, Dr. Khaja Zeeyauddin, Yagnambhatla Rajendra
Abstract :
Objective: A simple, Accurate, precise method was developed for the simultaneous estimation of the Sulfamethoxazole and Trimethoprim in pharmaceutical dosage form.
Methods: Chromatogram was run through Symmetry C18 (4.6 x 150mm, 5m). Mobile phase containing 70% MeOH: 30% phosphate buffer pH 4.6 was pumped through column at a flow rate of 1 ml/min. Buffer used at pH 4.6. Temperature was maintained at Ambient. Optimized wavelength for Sulfamethoxazole and Trimethoprim was 273 nm.
Results: Retention time of Sulfamethoxazole and Trimethoprim were found to be 2.003 min and 5.067 min. The % purity of Sulfamethoxazole and Trimethoprim was found to be 100.84 % and 100.29 % respectively. The system suitability parameters for Sulfamethoxazole and Trimethoprim such as theoretical plates and tailing factor were found to be 2711,1.6 and 3428, 1.3. the resolution was found to be 10.0. The linearity study for Sulfamethoxazole and Trimethoprim was found in concentration range of 25μg-125 μg and 25 μg-125 μg and correlation coefficient (r2) was found to be 0.999 and 0.999, % mean recovery was found to be 100.17 % and 100.24 %, %RSD for repeatability was 0.54 and 0.27 %. The precision study was precise, robust and repeatable. LOD value was 3.14 and 2.75, and LOQ value was 10.05 and 9.96 respectively.
Conclusion: The results of study showed that the proposed RP‐HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of Sulfamethoxazole and Trimethoprim in pharmaceutical dosage form.
Keywords: Sulfamethoxazole, Trimethoprim, RP‐HPLC, Simultaneous estimation.
Cite This Article:
Please cite this article in press Syed Luqman Al Jeelani Quadri et al., Method development and validation for the simultaneous estimation of Sulfamethoxazole and Trimethoprim by RP-HPLC,., Indo Am. J. P. Sci, 2022; 09(6).,
Number of Downloads : 10
References:
1. Sharma BK., “Instrumental Method of Analysis”; 27th Edn; Goel Publishing House, Meerut, 2011; 96-113.
2. “Quality Assurance of Pharmaceuticals”, (A summary of rules and related materials), Volume I, WHO, Geneva, 1997; 119-124.
3. Mendham J., Denny R.C., Barnes J.D., Thomas M. Vogels Text Book of Quantitative Chemeical Analysis; Pearson schooling, 2002; sixth Edn; 123-136.
4. ayar E, Sahin S, Cevheroglu S, Hincal AA. Development and validation of an HPLC method for simultaneous determination of trimethoprim and sulfamethoxazole in human plasma. Eur J Drug Metab Pharmacokinet. 2010 Sep;35(1-2):41-6.
5. Rønn AM, Mutabingwa TK, Kreisby S, Angelo HR, Fuursted K, Bygbjerg IC. A reversed-phase high-performance liquid chromatography method for the determination of cotrimoxazole (trimethoprim/ sulphamethoxazole) in children treated for malaria. Ther Drug Monit. 1999 Dec;21(6):609-14.
6. Gurumurthy.T*, Monika.M, Ashwini.V, Development and validation RP-HPLC method for simultaneous estimation of Sulfamethoxazole and Trimethoprim, Indian Journal of Research in Pharmacy and Biotechnology, 2017, 5(3): 235-238
7. Rajapandi, Raju & Resmi, V.R. & Venkateshan, N. & Babu, G.. (2016). Development and validation of RP-HPLC method for the simultaneous estimation of sulfadoxine and trimethoprim in bulk and pharmaceutical dosage forms. 8. 316-323.
8. Sayar, E., Sahin, S., Cevheroglu, S. et al. Development and validation of an HPLC method for simultaneous determination of trimethoprim and sulfamethoxazole in human plasma. Eur J Drug Metab Pharmacokinet 35, 41–46 (2010).