Volume : 09, Issue : 03, March – 2022

Title:

19.ISOLATION AND IDENTIFICATION OF MICRO ORGANISM FROM POND WATER AND ITS ANTIBACTERIAL EFFICACY AGAINST MORINGA OLIFERA

Authors :

Sangeetha. D *, Tharani. J, Ishwarya. R

Abstract :

The stagnant water reservoirs in urban area of India are severely contaminated for various purposes i.e. bathing, drinking and washing for humans and other animals. The contaminants due to surfactants, microbes, animals, nutrients, organic toxicants, etc. Some which can cause waterborne illness. Moringa olifera seeds extract was more effective than traditional antibiotics to combat the human pathogenic bacteria studied responsible for severe illness .The aim of the present study to isolate and identify the bacteria from pond water collected from Chidambaram , Annamalai Nagar .The microbial populations of the collected water sample were analyzed using standard plate count method .Based on the morphological and biochemical characterization the isolates were confirmed as Salmonella sp ,Pseudomonas sp ,Staphylococcus sp ,Escherichia coli ,Klebsiella sp ,Bacillus sp and Vibrio sp. The Moringa oleifera seed extracts were prepared using methanol, ethanol, acetone and aqueous extracts. The different crude extracts of Moringa oleifera were tested against all the isolated strains .Among the different extracts tested ,aqueous exhibited maximum zone of inhibition against Staphylococcus sp (27±0.2mm) followed by Salmonella sp (25±0.5) and Vibrio (25±0.1 )
KEYWORDS: Pond Water, Microorganism, Solvents, Moringa olifera seed, Crude extracts.

Cite This Article:

Please cite this article in press Sangeetha. D et al, Isolation And Identification Of Micro Organism From Pond Water And Its Antibacterial Efficacy Against Moringa olifera., Indo Am. J. P. Sci, 2022; 09(3),.

Number of Downloads : 10

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