Volume : 09, Issue : 05, May – 2022
Title:
63.STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF A NEW RP-HPLC TECHNIQUE FOR CALCULATING BULK AND PHARMACEUTICAL DOSE FORM OF ORLISTAT
Authors :
Syed Hafeez Ahmed, Anil kumar Middha
Abstract :
A simple, accurate and rapid RP-HPLC method has been developed for the estimation of Orlistat(ORL) in bulk and pharmaceutical dosage forms using a Zorbax C8 150mm×.4.6mm & 5.0µm particle size in isocratic mode, with mobile phase comprising of 0.1% formic acid :Actonitrile: Methanol in the ratio of 50:30:20 (v/v/v). The flow rate was 1ml/min and detection was carried out by UV detector at 215nm. The retention time for ORL was found to be 3.95 min. The calibration curve was obtained by plotting peak area versus the concentration over the range of 81-189 µg/mL and its percentage recovery was found to be 99.78-100.27%. The %RSD in the peak area of drug was found to be less than 2%. The number of theoretical plates was found to be more than 2000, which indicates efficient performance of the column.
Key words: Orlistat, HPLC, Isocratic, validation.
Cite This Article:
Please cite this article in press Syed Hafeez Ahmed et al, Stability Indicating Method Development And Validation Of A New Rp-Hplc Technique For Calculating Bulk And Pharmaceutical Dose Form Of Orlistat., Indo Am. J. P. Sci, 2022; 09(5).
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References:
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