Volume : 09, Issue : 10, October – 2022



Authors :

Nagesh Ramya, Kiruthika Balasubramanian *, Jayshree Srinivas

Abstract :

Background: For thousands of years, nature provides medicinal agents, and an incredible number of therapeutic drugs have been derived from natural sources, many based on their usage in traditional medicine. Objective: The goal of this study was to examine the antioxidant properties of Coleus vetteveroides leaves. Methodology: Enzymic antioxidants-POD, CAT, and GST, Non-enzymic antioxidants- ascorbic acid, flavonoids, tocopherol, chlorophyll, phenols, carotenoids, and lycopene. The free radical scavenging effects and chelating properties were observed by extracting the leaves with three different solvents (Hexane, Ethylacetate, and Isoproponol). Agarose gel electrophoresis was used to test the DNA protective activity of various leaf extracts against radicals. Result: The plant leaves exhibited strong antioxidant and radical scavenging properties. Conclusion: The leaves of Coleus vetteveroides have a potential content of both enzymatic and non-enzymatic antioxidants that could protect against oxidants and free radical damage. The phytochemical screening revealed the presence of flavonoids, alkaloids, and phenols.

Cite This Article:

Please cite this article in Kiruthika Balasubramanian et al, Free Radical Scavenging Properties Of Coleus vetteveroides Leaf Extracts And DNA Protection Against Hydrogen Peroxide Radical Damage: A Preliminary Analysis Of Enzymatic And Non-Enzymatic Antioxidants., Indo Am. J. P. Sci, 2022; 09(10).

Number of Downloads : 10


1. Paargavi SA, Aanandaroopan A, Priya TS, Merish S (2015) Review of Medicinal Plants Common to Traditional Chinese and Indian Medicines with Relevance to Non-Communicable Diseases. Current Traditional Medicine, 1:116-121
2. Frie, B. and Carr,C.(1993) vitamin C in human health and disease .J.Nutri Med., 8(13):14-16.
3. Silva and Ferandes A. (2010) Biological properties of medicinal plants: a review of their antimicrobial activity, The Journal of Venomous Animals and Toxins including tropical
Diseases, 16(3), 402- 413.
4. Sarma,A.S, Mallick, A.R. and Ghosh, A.K. (2010) Free radicals and their role in different clinical conditions: An overview, International Journal of Pharma Sciences and Research, 1(3)185-192.
5. Saranraj, P. and Sivasakthi, S. (2014) Medicinal Plants and its Antimicrobial Properties: A Review, Global Journal of Pharmacology 8 (3): 316-327.
6. Sindhe, A., Guru, J., and Naik, P., (2012) Effect of free radicals and antioxidants on oxidative stress: A review, Journal of Dental and Allied science; 1(2):63-66.
7. Hemalatha.M, Thirumalai, T., Saranya, R., Elumalai, E.K., and David, E. (2013) A Review On Antimicrobial Efficacy Of Some Traditional Medicinal Plants In Tamilnadu, Journal Of Acute Disease, 99-105.
8. Pryor, W.A. (2000) Dietery reference intake for vitamin C, vitamin E, selenium, and carotenoides. J.of Free Radic Chem.., 9(12):201-205.
9. Dhalla,N.S. Gupta,K.C. Sastry,M.S. and Malhotra (2000) The effect of stobadine on the copper induced peroxidation and its effects on cardiovascular diseases.,J,Rev-3.1152-1153.
10. Rahaman, I. The role of oxidative stress in pathogenesis and treatment of inflammatory bowel diseases.J.Naunyn Schmiedebergs Arch pharmacol., 387(7):605-620).
11. Rajmohan, S.B. and Gupta,N.T. (2008) Transduction with antioxidant enzyme catalase protects human T cells against oxidative stress. J. Immunol., 181(12):8382-8390.
12. Finkel, T. (1998) Oxygen radicals and signaling. Current Opinion in Cell Biology, 10, 248-253
13. Redza-Dutordoir M, Averill-Bates DA. Activation of apoptosis signalling pathways by reactive oxygen species. Biochim Biophys Acta. 2016 Dec;1863(12):2977-2992.
14. Pandey, K. B., & Rizvi, S. I. (2009). Plant polyphenols as dietary antioxidants in human health and disease. Oxidative medicine and cellular longevity, 2(5), 270–278.
15. Naz, R., & Bano, A. (2013). Phytochemical screening, antioxidants and antimicrobial potential of Lantana camara in different solvents. Asian Pacific Journal of Tropical Disease, 3(6), 480–486.
16. Saraswathy A, Lavanya MS (2013) Chemical Composition and Antibacterial Activity of the Essential Oil from the Roots of Coleus vettiveroides K.C. Jacob. International Journal of Pharmacy and Biological Sciences, 3(3)212-217
17. Luck, H. (1974), In: Methods in enzymatic analysis., Ber. Acad. N. Y., 885.
18. Reddy, K. P, Subhani, S. M., Khan, P. A. and Kumar (1995), Effect of light and benzyl adenine and dark- treated graving rice (Oryza sativa) leaves II. Changes in peroxidases activity, Plant. Cell. Physiol., 26, 987- 994.
19. Habig, W. H, Pabst, M. J. and Jokoby, W. (1974), The first enzymatic step in mercapturic acid III formation, J. Biol. Chem., 249, 7130- 7139.
20. Roe, J. H. and Keuther, C. A. (1943), The determination of ascorbic acid in whole blood and urine through 2, 4-dinitro phenyl hydrazine derivative of dehydro ascorbic acid, J. Biol. Chem., 147, 399- 407.
21. Rosenberg, H.R. (1992), Chemistry and physiology of the vitamins, Intersci. Pub, N.Y., 452- 453
22. Zakaria, H., Simpson, K., Brown, P. R. and Krotulovic, A. (1979), Use of reversed phase HPLC analysis for the determination of provitamin A carotenes in tomatoes, J. Chrom, 176, 109- 117.
23. Mallick, C. P. and Singh, M. B. (1980), Plant enzymology and plant histoenzymology, Kalaya. Pub., New. Delhi., 281- 286.
24. Cameron, G.R., Mitton, R.F. and Allan, J.W. (1943), Lancet, 179.
25. Moron, M.S., Depierre, J.N. and Mannervik, Y.C. (1979), Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver, Biochem. Biophys. Acta., 582, 67-68.
26. Witham, F.H., Blaydes, B.F. and Devlin, R.M. (1971), Experiments in plant physiology, Van Nostrand-Reinhold. 245.
27. Ruch, R.J., Cheng, S. and Klaunig, J.E. (1989) Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea, Carcinogenesis, 10, 1003-1008.
28. Elizabeth, K. and Rao, H.N.A. (1990) Oxygen radical scavenging activity of curcumin, Int. J. Pharm., 58, 237-240.
29. Brown, D.J. and Goodman, J. (1998), A review of vitamin A, C and E and their realationship to cardiovascular disease, Clin. Excell. Nurse Pract., 2, 10-12.
30. Marmur,J. (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms, journal of Molecular Biology,3(2),208-218.
31. Khandelwal, K.R. (2002) Practical pharmacognosy-techniques and experiments, IX edition, NiraliPrakashan Publishers, Pune, pp. 149-157.
32. Verma, S. and Dubey, R.S. (2003) Lead Toxicity Induces Lipid Peroxidation and Alters the Activities of Antioxidant Enzymes in Growing Rice Plants. Plant Science, 164, 645-655
33. Gomathinayagam M, Jaleel CA, Lakshmanan GM, Panneerselvam R. (2007) Changes in carbohydrate metabolism by triazole growth regulators in cassava (Manihot esculenta Crantz); effects on tuber production and quality. C R Biol. 330(9):644-55.
34. Sankhla, N., Upadhyaya, A., Davis, T.D. et al. (1992) Hydrogen peroxide-scavenging enzymes and antioxidants in Echinochloa frumentacea as affected by triazole growth regulators. Plant Growth Regulations, 11, 441–443
35. Jaleel, C.A., Riadh, K., Gopi, R. et al. (2009) Antioxidant defense responses: physiological plasticity in higher plants under abiotic constraints. Acta Physiol Plant 31, 427–436
36. Nirmaladevi R, Padma PR (2008) A study on the antioxidant potential of three under-exploited plants of medicinal value. Plant Archives. 8(1):339-341
37. Starlin T, Gopal Krishna VR (2013) Enzymatic and non-enzymatic antioxidant properties of Tylophora pauciflora Wight and Arn.–An in vitro study. Asian Journal of Pharmaceutical and Clinical Research. 6(4): 68-71
38. Zimmermann P, Zentgraf U (2005) The correlation between oxidative stress and leaf senescence during plant development. Cellular and Molecular Biology Letters.;10(3):515-34
39. Shih, M. C., Chang, C. M., Kang, S. M., & Tsai, M. L. (2011). Effect of different parts (leaf, stem and stalk) and seasons (summer and winter) on the chemical compositions and antioxidant activity of Moringa oleifera. International journal of molecular sciences, 12(9), 6077–6088
40. Serteser A, Kargioğlu M, Gök V, Bağci Y, Ozcan MM, Arslan D (2008) Determination of antioxidant effects of some plant species wild growing in Turkey. International Journal of Food Sciences and Nutrition. 59(7-8):643-51
41. Ching LS, Mohamed S. (2001) Alpha-tocopherol content in 62 edible tropical plants. Journal of Agricultural and Food Chemistry. 49(6):3101-5.
42. Velioglu YS, Mazza G, Gao L, Oomah BD (1998) Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products. Journal of Agricultural and Food Chemistry 46(10):4113-4117
43. Krishna RK, Krishnakumari S, Chandrakala S (2012) Evaluation of antioxidant properties of different parts of Amorphophallus commutatus, an endemic aroid of Western Ghats, South India. International Journal of Pharma and Bio Sciences 3(3):B443-B455
44. Karthika P, Hema Karthika R, Jayachandran N, Joice Rathinam E (2014) Estimation of Chlorophyll Content in Papaya Leaf using Mathematical Operations. International Journal of Computer Applications 6-11
45. Lipinski B (2011) Hydroxyl Radical and Its Scavengers in Health and Disease, Oxidative Medicine and Cellular Longevity (5):809696
46. Keser S, Celik S, Turkoglu S, Yilmaaz O (2012) Hydrogen Peroxide Radical Scavenging and Total Antioxidant Activity of Hawthorn, Chemistry Journal 2(1):9-11
47. Peiyuan Li, Lini Huo, Wei Su, Rumei Lu (2011) Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica, Journal of the Serbian Chemical Society 76(5):709-717
48. Peter Y.Y. Wong, David D Kitts (2006) Studies on the dual antioxidant and antibacterial properties of Parsley (Petroselinum crispum) and Cilantro (Coriandrum sativum) Extracts, Food Chemistry 97(3):505-515
49. Wang TS, Liang SJ, Kueii Li C, Yie Liu S (2004) Protective Effect of Water Yam (Dioscorea alata L.) Extract on the Copper-driven Fenton Reaction and X-ray Induced DNA Damage in vitro, Phytotherapy Research 18(4):325-8
50. Ningappa MB, Ramdas D, Srinivas L (2008) Antioxidant and free radical Scavenging activities of polyphenol-enriched curry leaf (Murrayakoenigii L.) extracts, Food Chemistry 106(2)
51. Ramya N, Nagarathna A, Kiruthika B (2020) Antioxidant Potential of Adathoda vacia leaves, World Journal of Pharmacy and Pharmaceutical Science 9(4): 1426-34
52. Ramya N, Nagarathna A, Kiruthika B (2020) Phytochemical Screening and Protective Effect of Adhatoda Vasica Leaf Extracts against Free Radical Damage, Indo American Journal of Pharmaceutical Sciences 7(9): 691-7
53. Swathi KA, Nagarathna A, Kiruthika B (2020) Antioxidant analysis of Carissa carandas linn plant leaves, , Indo American Journal of Pharmaceutical Sciences 7(4):980-985
54. Swathi KA, Nagarathna A, Kiruthika B (2020) Radical Scavenging, Preliminary Phytochemical Screening and DNA Protective effect of Carissa Carandas Linn Plant Leaves, International Journal of Pharma and Bio Sciences 12(1):1-8