Volume : 09, Issue : 09, September – 2022



Authors :

Manmohan Patel*, Archana Tiwari, P. K. Dubey

Abstract :

Four methods RP–HPLC and UV–Spectrophotometry (Zero Order – AUC, First Order Derivative using Amplitude and First Order Derivative using AUC) have been developed for determination of Midodrine Hydrochloride in bulk and tablets. RP – HPLC method are found to be accurate, precise, rugged and sensitive. A simple, sensitive, precise and accurate RP – HPLC method for the determination of Midodrine Hydrochloride both bulk and pharmaceutical formulation has been developed and validated as per the International Conference on Harmonization (ICH) guidelines. The % RSD value for intra – day and inter – day precision was found to be in the range of 0.45 – 1.37 % and 1.48 – 1.55 %. The mean % recovery was found to be in the range of 98.87 – 99.59 %. The low values of LOD (0.09 µg) and LOQ (0.28 µg) indicate high sensitivity of the method. The developed method can routinely be used for analysis of Midodrine Hydrochloride in pharmaceutical formulations. Three simple, rapid, accurate, precise, reliable and economical UV – Spectrophotometry methods have been proposed for the determination of Midodrine Hydrochloride in bulk and in pharmaceutical formulation. Method 2 is zero order UV – Spectrophotometry using Area Under Curve Method, Method 3 is first order derivative UV – Spectrophotometry using Amplitude and Method 4 first order derivative UV – Spectrophotometry using Area Under Curve Method. The developed methods have shown good results in terms of linearity, accuracy, precision and sensitivity for bulk drug and marketed formulation as well.
Key words: Midodrine hydrochloride, HPLC, UV-Spectrophotometry, Method Development, validation

Cite This Article:

Please cite this article in Manmohan Patel et al, Quantitative Estimation Of Midodrine Hydrochloride By RP-HPLC And UV-Spectrophotometry Methods., Indo Am. J. P. Sci, 2022; 09(9).

Number of Downloads : 10


1. Christian G. D., Analytical Chemistry, 6th Edn. Wiley International, 2014, 604.
2. Gennaro A. R., Remington, The science and Practice of Pharmacy, Indian edition, 20th edn, Lippincott Williams and Wilkins, Vol – I, 2010; pp. 587-610.
3. Skoog D. A., F. J. Holler and Nieman T. A., Principles of Instrumental Analysis,Thomson Brook/cole, 5th edn, 2015 ; pp. 674-696.
4. Katz E., Quantitative Analysis Using Chromatographic Technique, Wiley India Pvt.
5. Sethi P. D., Introduction – High Performance liquid Chromatographic, 1st edn, CBS Publishers, New Delhi, 2019, pp. 1 – 28.
6. Swadesh J., HPLC – Practical and Industrial Applications – CRC Press, Boca Raton, 2007, pp. 20 – 25
7. Ananda K, Kavitha MP, Krishnakumar K. A review on the determination of Midodrine hydrochloride in bulk and marketed formulations by using different analytical techniques.2020; 7(14): 260-266.
8. ICH-Guidelines Q2 (R1), Validation of Analytical Procedures: Text and Methodology. (2005)