Volume : 10, Issue : 01, January – 2023

Title:

14.EXTRACTION PHYTOCHEMICAL SCREENING AND TLC OF MEDICINAL PLANT EXTRACTS (PSIDIUM GUAJAVA)

Authors :

Relam Dawar, Dr. Vivekanand Katare*, Mr. Shriram Sen, Dr. Prabhat Kumar Jain

Abstract :

Psidium guajava L, belonging to the Myrtacea family, has been reported to have anti-diarrheal, hepatoprotective, hypoglycemic, lipid lowering, antibacterial and antioxidant activities. It contains important phytoconstituents such as tannins, triterpenes, flavonoid: quercetin, pentacyclic triterpenoid: guajanoic acid, saponins, carotenoids, lectins, leucocyanidin, ellagic acid, amritoside, beta-sitosterol, uvaol, oleanolic acid and ursolic acid. Thin layer chromatography has become widely used method for the separation of substances in a great variety of inorganic and organic studies. This investigation aimed to perform the extraction, phytochemical screening and TLC of Psidium guajava. The results showed that the yields were found to be (7.39% w/w of crude drug) of Methanolic extract Psidium guajava leaves. Obtained results were recorded in table 1. Results of Phytochemical test showed the presence of Carbohydrates, Flavonoids, Proteins & Amino acids, Diterpenes, Saponins, Alkaloid and phenols (Table 2). Total Flavonoid content was 0.165mg/100mg quercetin equivalent in extract. The TLC was also performed to identify the presence of favanoids.It shows that the presence of such bioactive compounds has medicinal importance. Hence Psidium guajava was found to have medicinal properties.
Key words: Herbal Medicine, Psidium guajava, Thin Layer Chromatography, Flavonoid

Cite This Article:

Please cite this article in press Vivekanand Katare et al Extraction Phytochemical Screening And Tlc Of Medicinal Plant Extracts (Psidium Guajava).., Indo Am. J. P. Sci, 2023; 10(01).

Number of Downloads : 10

References:

1. Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in pharmacology. 2014 Jan 10;4:177.
2. Gutiérrez RM, Mitchell S, Solis RV. Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. Journal of ethnopharmacology. 2008 Apr 17;117(1):1-27.
3. Culberson CF, Culberson WL, Johnson A. A standardized TLC analysis of β-orcinol depsidones. Bryologist. 1981 Apr 1:16-29.
4. Pandey, A., & Tripathi, S. (2014). Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry, 2(5).
5. Ojewunmi, O. O., Oshodi, T., Ogundele, O. I., Micah, C. & Adenekan, S. In vitro antioxidant, antihyperglycaemic and antihyperlipidaemic activities of ethanol extract of Lawsonia inermis leaves. Br. J. Pharm. Res. 4(3), 301–314 (2014).