Volume : 10, Issue : 01, January – 2023
20.CHARACTERIZATION EVALUATION AND IN VITRO ANTIOXIDANT TESTING OF PSIDIUM GUAJAVA (STEM)
Ramkishan Kushvaha, Dr. Vivekanand Katare*, Mr. Shriram Sen, Dr. Prabhat Kumar Jain
Herbal therapy, an oldest kind of medicare known to humans and involves the use of entire plant or plant part, for the treatment of various debilitating diseases or to support good health Natural herbs were extensively used for the treatment and prevention of various ailments since ancient times. Guava (Psidium guajava Linn.) Family Myrtaceae is important plant used traditionally for medicinal purpose Psidium guajava, is an important food crop and medicinal plant in tropical and subtropical countries is widely used like food and in folk medicine around of the world The pharmacological actions and the medicinal uses of aqueous extracts of guava leaves in folk medicine include the treatment of various types of gastrointestinal disturbances such as vomiting, diarrhea, inhibition of the peristaltic reflex, gastroenteritis, spasmolytic activity, dysentery, abdominal distention, flatulence and gastric pain. This study focuses on Characterization evaluation and in vitro antioxidant testing of Psidium guajava Stem. The results showed percentage yield of methanolic extract of Psidium guajava as 13.6%. The results of the phytochemical test indicated the presence of phenols, Diterpenes, proteins and amino acids, flavonoids, and carbohydrates. The TLC Profile results indicated the presence of flavonoids, a bioactive molecule with a variety of pharmacological actions. The DPPH method for determining antioxidant potential was performed. The IC50 value obtained for methanolic extract of Psidium guajava was 65.31%. From above obtained results it can be concluded that the stem of Psidium guajava have potent antioxidant potential.
Keywords: Psidium guajava, Anti-oxidant, TLC, IC50
Cite This Article:
Please cite this article in press Vivekanand Katare et al, Characterization Evaluation And In Vitro Antioxidant Testing Of Psidium Guajava (Stem).., Indo Am. J. P. Sci, 2023; 10(01)./p>
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1. Namjooyan F, Ghanavati R, Majdinasab N, Jokari S, Janbozorgi M. Uses of complementary and alternative medicine in multiple sclerosis. J Tradit Complement Med. 2014;4(3):145-52. doi: 10.4103/2225-4110.136543, PMID 25161918.
2. Payyappallimana U. Role of traditional medicine in primary health care: An overview of perspectives and challenging; 2010
3. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH: Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal 2006; 19: 669–675.
4. Koo MH, Mohamed S: Flavonoid (myricetin, quercetin, kaempferol, luteolin and apigenin) content of edible tropical plants. J Agric Food Chem 2001; 49: 3106–3112.
5. Mittal P, Gupta V, Kaur G, Garg AK, Singh A. Phytochemistry and pharmacological activities of Psidium guajava. IJPSR. 2010;1(9):9-19.
6. Kokate CK. Practical Pharmacognosy. 4th edition. Delhi: Vallabh Prakashan; 1994.
7. Harborne JB. Phytochemical methods. London: Chapman and Hall; 1973
8. Sherma, J., & Fried, B. (Eds.). (2003). Handbook of thin-layer chromatography. CRC press.
9. Olufunmiso OO, Afolayan AJ, Phenolic content and antioxidant property of the bark extract of Ziziphus mucronata willd. Subsp. mucronata willd, BMC Complement Alter Med 2011; 11:130