Volume : 10, Issue : 07, July – 2023
Title:
43.ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF NORTRIPTYLINE AND PREGABALIN PHARMACEUTICAL DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (RP-HPLC)
Authors :
Shabana Sultana, Dr. R. Vani, Dr. D.Ramakrishna
Abstract:
A simple and selective LC method is described for the determination of Pregabalin and Nortriptyline in tablet dosage forms. Chromatographic separation was achieved on a C18 column using mobile phase consisting of a mixture of 50 volumes of methanol and 50 volumes of phosphate buffer with detection of 255 nm. Linearity was observed in the range 60-140 μg /ml for Pregabalin (r2 =0.997) and 3-7μg /ml for Nortriptyline (r2 =0.995) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. From the above experimental results and parameters it was concluded that, this newly developed method for the simultaneous estimation of Pregabalin and Nortriptyline drugs was found to be simple, precise, accurate and high resolution and shorter retention time makes this method more acceptable and cost effective and it can be effectively applied for routine analysis in research institutions, quality control department in meant in industries, approved testing laboratories studies in near future.
KEYWORDS: Pregabalin , Nortriptyline , Chromatographic separation , Drug development.
Cite This Article:
Please cite this article in press Shabana Sultana et al Analytical Method Development And Validation For Simultaneous Estimation Of Nortriptyline And Pregabalin Pharmaceutical Dosage Form By Using Reverse Phase High Performance Liquid Chromatography (RP-HPLC), Indo Am. J. P. Sci, 2023; 10 (07).
Number of Downloads : 10
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