Volume : 10, Issue : 03, March – 2023
Title:
29.ANALYSIS OF CALCIFEROL AND ELEMENTS IN AQUEOUS EXTRACT OF MUNTIGIA CALABURA FRUIT USING LC-MS/MS AND ICPMS TECHNIQUES
Authors :
K.B. Leneeygreen* K. Rasika, K. Rajathi
Abstract :
Muntigia calabura fruit have a lot of medicinal properties like antimicrobial, antioxidant, anti-inflammatory, and anticarcinogenic activities. It is used to reduce headaches, chronic pain, arthritis, blood pressure, prevent infection and boost immunity. This study planned to find out the amount of Calciferol, Cu, Zn and Selenium present in Muntigia calabura fruit. Aqueous fruit extract were prepared and analyzed by above mention tests using LCMS/MS and ICPMS analytical chemistry techniques. These both techniques are very advanced and assist to find out the accurate quantitative results. The sample preparation, analyzer method and calculations are carried out with reference to AOAC protocol. In this fruit extract contains 0.91µg/100g of Calciferol, 2.22mg/kg of Copper, 0.1mg/kg quantity of Selenium and Zinc metals were observed.
Key words: Copper, Zinc, Selenium, LCMS/MS, ICPMS, Calciferol, Muntigia calabura
Cite This Article:
Please cite this article in press K.B. Leneeygreen et al, Analysis of Calciferol And Elements In Aqueous Extract Of Muntigia Calabura Fruit Using LC-MS/MS and ICPMS Techniques, Indo Am. J. P. Sci, 2023; 10 (03).
Number of Downloads : 10
References:
1. Aulia Nur Rahmawati, Okid Parama Astirin Artini Pangastuti, Intracellular antioxidant activity of Muntingia calabura leaves methanolic extract, Nusantara Bioscience. Vol. 10, No. 3, pp. 210-214.
2. Vijayanand S and Ann Steffy Thomas, Screening of Muntingia Calabura and Theobroma Cacao for Potential Bioactives. International Journal of Recent Scientific Research Research Vol. 9, Issue, 1(B), pp. 22991-22996, January, 2018.
3. Ibrahim Abdel Aziz Ibrahim, Mahmood Ameen Abdulla, Siddig Ibrahim Abdelwahab, Fouad Al-Bayaty and Nazia Abdul Majid. Leaves Extract of Muntingia Calabura Protects Against Gastric Ulcer Induced by Ethanol in Sprague-Dawley Rats. Journal of Clinical and Experimental Pharmacology, 2012, S5.
4. S.V. Saibaba, M. Sathish Kumar and P. Shanmuga Pandiyan, Mini review on LC/MS techniques, World journal of pharmacy and pharmaceutical sciences, Volume 5, issue 4, 2381-2395.
5. Hiroshi Kawaguchi, Inductively coupled Mass Spectrometry A Review, Analytical Sciences, vol 4, 1988.
6. Adrian A. Ammann, Inductively coupled plasma mass spectrometry (ICP MS): a versatile tool, J. Mass Spectrom. 2007; 42: 419–427.
7. Dr.S.Vadivelan, Jebaseelan, B. Edwin Jose, Dr.R.Meera, S.Manikandan, Dr.R.Kalirajan Dr.T.Sarojini, Phytochemical Investigation and Antimicrobial Activities of Leaf Extract of Muntingia calabura Linn, Int. J. Pharm. Sci. Rev. Res., 69(2), July – August 2021; Article No. 06, Pages: 42-46.
8. AOAC Official Method 2016.05Analysis of Vitamin D2 and Vitamin D3in Fortified Milk Powders, Infant Formulas, and Adult/ Pediatric Nutritional Formulas Liquid Chromatography–Tandem Mass Spectrometry. (AOAC 2016.5)