Volume : 10, Issue : 05, May – 2023
Title:
25.ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR QUANTITATIVE ESTIMATION OF ANTIPSYCHOTIC DRUG IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD
Authors :
Hirachand Narayankar, V M Waghulkar, A W Baitule
Abstract :
Abstract:
Analytical method was developed for the estimation of Risperidone drug substance by High Performance liquid chromatography. The chromatographic separation was achieved on C8 column (Zodiac 100, 150×4.6mm, 5um) at ambient temperature, the separation achieved employing a mobile phase consists of 0.1%v/v Ammonium formate in water: Methanol (30:70). The flow rate was 1.0 ml/ minute and ultra violet detector at 240nm. The average retention time for Risperidone found to be 3.52 min the proposed method was validated for selectivity, precision, linearity and accuracy. All validation parameters were within the acceptable range. Antipsychotic drug tend to block dopamine D2 receptor in dopaminergic pathways of brain this means that dopamine released in these pathway has less effect. Excess release of dopamine in the mesolimbic pathway has been linked to psychotic experience decreased dopamine released in other pathway are associated with psychotic episodes in schizophrenia and bipolar disorder.
Keywords:- Risperidone, HPLC, C8, Ammonium formate, Validation etc.
Cite This Article:
Please cite this article in press Hirachand Narayankar et al, Analytical Method Development And Validation For Quantitative Estimation Of Antipsychotic Drug In Pharmaceutical Dosage Form By RP-HPLC Method., Indo Am. J. P. Sci, 2023; 10 (05).
Number of Downloads : 10
References:
1. Ashutosh Kar, Pharmaceutical Analysis-I, New International Ltd. Publishers, 1st Edition, 2020.
2. Tripathi Medical Pharmacology, 8th edition New Delhi jaypee brothers medical Published 2019: 462-464.
3. Sharma B. K., Instrumental Methods of Chemical Analysis, 19th Edition, Meerut: Krishna Prakashan Media (P) Ltd., 1 January 2014, Page No. 1-4.
4. Kenneth A. Connors, A Textbook of Pharmaceutical Analysis. 3rd Edition, Published by John Wiley & Sons, 1967, Page No.1-3.
5. Chatwal G. And Anand K., Instrumental Method of Chemical Analysis, 5th Edition Himalaya Publishing House Mumbai, 2007.
6. S Koog, D. A. Holler, F.J. Timonthy, A. Nieman, Principle of Instrumentation Analysis, 5th Edition, Sauders College Publication, London 2004, Page No. 621-659.
7. Aguilar M-1, editor, HPLC of Peptides and Proteins. Est ed. New Jersey: Humana Press; 2004; 9-22 p.
8. Ahuja S. Rasmussen H, editors. Hplc Method Development For Pharmaceuticals. 2007th ed. Elsevier Inc.: 2007, P. 1-11.
9. https://pubchem.ncbi.nlm.nih.gov/compound/Risperidone
10. https://en.wikipedia.org/wiki/risperidone