Volume : 10, Issue : 05, May – 2023
Title:
46.METHOD DEVELOPMENT, VALIDATION AND OPTIMIZATION FOR SIMULTANEOUS ESTIMATION OF CELECOXIB AND PIPERINE BY RP-HPLC
Authors :
Ajinkya R. Malpe, Dr. Monika P.Jadhao
Abstract :
It is essential to study the stability of celecoxib in presence of piperine. The stability analysis of the drugs individually (celecoxib/piperine) and in combination (celecoxib with piperine) was carried out at different pH conditions (4.5 and 7.4 pH). Reverse Phase High Performance liquid chromatography method was developed for celecoxib and piperine in combined form. The method was optimized and developed using the design of experiments (DOE). For the optimization, the Design expert trial version 13 was used. The developed method was analyzed using Zodiac C-8 Luna, 5 µm, 100Å, and 150 mm column and methanol: acetonitrile (30:70) with 0.1% of trifluoroacetic acid as mobile phase (1 ml/minute, flow rate) and detected at a wavelength of 342 nm for piperine and 253 nm for celecoxib. Retention time of piperine and celecoxib was found to be 4.751 and 6.685 minutes respectively. The correlation coefficient of both drugs was found to be 0.999. The accuracy of piperine was found to be 99.07-100.61% whereas for celecoxib, it was 99.90-100.31. Overall % RSD was found to be less than 2%. Results showed that the celecoxib stability decreases slightly in the presence of piperine. The method was validated according to the International Conference on Harmonization guidelines and found to be reproducible with peaks showing good resolution with short retention time and can be used for simultaneous estimation of of celecoxib and piperine.
Cite This Article:
Please cite this article in press Ajinkya R. Malpe et al,. Method Development, Validation And Optimization For Simultaneous Estimation Of Celecoxib And Piperine By RP-HPLC., Indo Am. J. P. Sci, 2023; 10 (05).
Number of Downloads : 10
References:
1. Joyceline P, Bharath R, Simultaneous estimation of paclitaxel and curcumin in nanoformulation; Stablility analysis of drugs,optimization and validation of HPLC method, Journal of Applied Pharmaceutical Science, 2021; Vol. 11(03), pp 071-083.
2. Haijun X, Sedlarik V, A Rapid and Sensitive HPLC Method for Simultaneous Determination of Irinotecan Hydrochloride and Curcumin in Co-delivered Polymeric Nanoparticles, Journal of Chromatographic Science, 2020; Vol.58, No.7, 651-660.
3. Dong Wuk Kim, Abid Mehmood, Yousaf. et al, Development of RP-HPLC method for simultaneous determination of docetaxel nad curcumin in rat plasma: validation and stablility, Asian Journal of Pharmaceutical Sciences, 2016; S1818-0876 (16)30073-3.
4. Kaur Sukhbir, Kaur Lavleen, Spectrophotometric method for simultaneous estimation of ornidazole and curcumin in pure form, The Pharma Innovation Journal, 2014; 3(5): 01-04.
5. Kalra R .et al, Simultaneous estimation of Artemether and curcumin by RP-HPLC method, Pharmacophore, 2016; Vol. 7 (3), 141-151.
6. Desai N, Momin M, Singh U.et al, Analytical method development and validation for simultaneous estimation estmation of curcumin and cyclosporine by RP-HPLC, International Journal of Pharmacy and Pharmaceutical Sciences, 2019; Vol 11, Issue2, 0975-4491.
7. Ankalgi D, Chaudhary N.et al, Method development and validation for simultaneous estimation of methotrexate and curcumin in Bulk Drug by using RP-HPLC, Journal of Drug Delivery and Therapeutics, 2019; 9(6-s):127-135.
8. Panigrahi S, Hirlekar R, A new stability indicating RP-HPLC method for determination of curcumin : An application to nanoparticulate formulation, International Journal of Pharmacy and Pharmaceutical Sciences, 2016; Vol 8, Issue 12,0975-1491.
9. Patil N, Mahajan H, Development and validation of RP-HPLC method for simultaneous estimation qualitative and quantitative estimation of curcumin and quercetin in bulk mixture, Indian Journal of Pharmaceutical Education and Research, 2022; Vol 56,Issue 1.
10. Khismatrao A, Bhairy S, Hirlekar R, Development and validation of RP-HPLC method for simultaneous estimation of curcumin and piperine, International Journal of Applied Pharmaceutics,2019; Vol 10, Issue 5,0975-7058.
11. Edlabadkar A, Rajput A, QbD Approach –RP HPLC method for optimization ,development and validation of garenoxacin mesylate in bulk and in tablets, Eurasian Journal of Analytical Chemistry, 2018; 13(4), em26.
12. Gosavi S, Kasar S .et al, Development and validation of RP-HPLC and HPTLC method for estimation of curcumin in hiridrakhand polyherbal tablet formulation, Journal of Pharmacognosy and Phytochemistry, 2019; 8(3): 2562-2568.
16. Ahmad S, Ahire S, Tatiya U, QbD Approach Method Development and Validation for the Simultaneous Estimation of Methotrexate and Folic acid by UV Spectrophometric and RP-HPLC in Bulk and Tablet Dosage Forms, Journal of Pharmaceutical Science & Research, 2021; Vol. 13(9), 553-558.
17. Atodaria E, Mashru R, Development and Validation of Some Classical and Novel Spectrophotometric Methods and RP-HPLC Method by QbD Approach for Simultaneous Estimation of Aripiprazole and Clozapine in Synthetic Mixture, World Journal of Pharmaceutical Research, 2018; Volume 7, Issue 10, 521-557.
18. A.K.Palakruthi .et al, QbD based development of HPLC method for simultaneous quantification of Telmisartan and Hydrochlorothiazide impurities in tablets dosage form, Practical Laboratory Medicine, 2020; 21-e00169.
19. Pratiksha S.et al, QbD Approach for Stability Indiacting HPLC Method for Determination of Artemether and Lumafantrine in Combined dosage form, International Journal of Drug Regulatory Affairs, 2017; 5(4), 44-59.