Volume : 11, Issue : 08, August – 2024
Title:
DEVELOPMENTAND VALIDATION OF SPECTROSCOPIC SIMULATANEOUS EQUATION METHOD FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE AND VALSARTAN IN PURE AND PHARMACEUTICAL DOSAGE FORM
Authors :
Sameena Begum*,Venkatapally Tejaswini,Sunaiya Tazeen,Heera Bai, B.Akanksha, Shagufta Noureen
Abstract :
The Analytical method was developed by Studying Different Parameters. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)).First of all, Maximum absorbance was found to be At 220 nm for Amlodipine, 252nm for Valsartan. And peaks were excellent.The UV spectrum of Amlodipine, Valsartan were obtained by using Methanol as a solvent and then validated.Concentration was selected to be 10µg/ml for Amlodipine, Valsartan which gave good Peaks.The linearity study for Amlodipine, Valsartan were found in concentration range of 2µg-10µg and correlation coefficient (r) was found to be 0.99 for amlodipine, Valsartan. And Regression coefficient (r2) 0.99 for amlodipine, Valsartan.%Recovery studies were carried out and the percentage recovery was found to be in the range of 100% – 101% for Amlodipine, 100.65% to 101% for Valsarn %RSD of Absorbance for Intraday, Inter day precision was found to be 0.66 and 0.99 for Amlodipine, 0.88,0.88 for Valsartan respectively % RSD for Ruggedness was found to be less than 2. LOD and LOQ of calibration curve of drug prepared in Methanol .And concentration was found to be 1 and 3.14µg/ml respectively for Amlodipine.1 and 3.15 µg/ml respectively for Valsartan Hence the suggested UV Spectrophotometric method can be used for routine analysis of Glimepiride in API and Pharmaceutical dosage form
Key Words; ICH guidelines (ICH, Q2 (R1)), Amlodipine, Valsartan,Regression coefficient (r2) and calibration curve of drug prepared in Methanol.
Cite This Article:
Please cite this article in press Sameena Begum et al Development and Validation Of Spectroscopic Simultaneous Equation Method For Simultaneous Estimation Of Amlodipine And Valsartan In Pure And Pharmaceutical Dosage Form.,Indo Am. J. P. Sci, 2024; 11 (8).
Number of Downloads : 10
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