Volume : 11, Issue : 12, December – 2024
Title:
DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS DETERMINATION OF DECITABINE AND CEDAZURIDINE BY RP-HPLC METHOD
Authors :
R.Malavila , P.Aravindra Reddy
Abstract :
Introduction: An attempt has been made to develop a validated stability indicating RP-HPLC method for the estimation of decitabine and cedazuridine. Literature survey revealed that many analytical methods have been reported individually or in combination with other drugs. Retention times were decreased and that run time was decreased, so the method developed was simple and economical.It includes the general information on RP-HPLC and method development, general information on forced degradation studies and stress conditions like acid, base, peroxide, thermal, photolytic and neutral. Discussion: The article discusses about drug profiles and official status of selected drugs i.e., decitabine and cedazuridine. Results: In this article the previous literature available for drugs is used for developed research work which Include stability indicating RP-HPLC method development and validation for simultaneous estimation of decitabine and cedazuridine in bulk and their pharmaceutical dosage form. Using Waters HPLC 2695 system, quaternary gradient pump equipped with auto sampler injector with 20 μL is injected eluted with the mobile phase containing 65% 0.01 N KH2 PO4: 35% acetonitrile which is pumped at a flow rate of 1 mL/min and detected by PDA detector at 245 nm. The peak of decitabine and cedazuridine was eluted at retention times of 2.263 min and 3.001 min, respectively. Conclusion: In this paper, HPLC method for the selected drugs showed good linearity.
Keywords: Decitabine, Cedazuridine, RP-HPLC
Cite This Article:
Please cite this article in press P.Aravindra Reddy et al., Development And Validation For Simultaneous Determination Of Decitabine And Cedazuridine By RP-HPLC Method.,Indo Am. J. P. Sci, 2024; 11 (12).
Number of Downloads : 10
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