Volume : 11, Issue : 12, December – 2024
Title:
METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF FLUCONAZOLE AND TINIDAZOLE IN BULK AND PHARMACEUTICAL DOSAGE FORMS BY RP-HPLC
Authors :
T.Venkatesh, M.Chaitanya, P.Aravindra Reddy
Abstract :
RP-HPLC method was developed and validated as per ICH guidelines for the estimation of Fluconazole and Tinidazole. Simultaneous Estimation of Fluconazole and Tinidazole were carried out by RP- HPLC using Ortho phosphoric acid buffer & methanol, and column Inertsil C18 (250*4.6 mm, 5um) as a stationary phase and peak was observed at 213 nm which was selected as a wavelength for quantitative estimation. After the development of the method, it was validated for various parameters. It was found that recovery value of pure drug was between 99 % to 101% which indicates that the method is accurate and also reveals that commonly used excipients and additives present in the pharmaceutical formulations were not interfering in the proposed methods. The ruggedness of the method was checked by different analysts and found that the results were nearly same which indicates that the method is rugged. Based on the results observed, it was concluded that proposed method can be used for routine analysis of fluconazole and tinidazole.
Key words: Fluconazole, Tinidazole anti-protozoal agent, linearity, accuracy, precision.
Cite This Article:
Please cite this article in press M.Chaitanya et al .,Method Development And Validation For Simultaneous Estimation Of Fluconazole And Tinidazole In Bulk And Pharmaceutical Dosage Forms By Rp-Hplc.,Indo Am. J. P. Sci, 2024; 11 (12).
Number of Downloads : 10
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