Volume : 11, Issue : 12, December – 2024
Title:
METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF NEBIVOLOL AND HYDROCHLOROTHIAZIDE BY USING RP-HPLC METHOD
Authors :
T.Kavitha Rani,M.Chaitnaya , P.Aravindra Reddy
Abstract :
High Performance Liquid Chromatography is at present one of the most sophisticated tools of the analysis. The estimation of Nebivolol, Hydrochlorothiazide was done by RP-HPLC. The Phosphate buffer of pH 3.0 and the methanol as mobile phase was optimized with consists of Phosphate buffer and ACN (40: 60% v/v) Thermo Hypersil BDS, C18 and equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using UV detector at 282 nm. The solutions were chromatographed at a constant flow rate of 0.8 ml/min. The linearity range of of Nebivolol and Hydrochlorothiazide were found to be from 6.25-37.5g/ml and 2.5-15g/ml of. Linear regression coefficient was found to be less than 0.999. The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 98-102% of Nebivolol and Hydrochlorothiazide. LOD and LOQ were found to be within limit. The results obtained on the validation parameters met ICH and USP requirements. It inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application in routine laboratory analysis with high degree of accuracy and precision.
Key words: Nebivolol, Hydrochlorothiazide, Validation, Linearity.
Cite This Article:
Please cite this article in press M.Chaitnaya et al., Method Development And Validation For The Estimation Of Nebivolol And Hydrochlorothiazide By Using Rp-Hplc Method.,Indo Am. J. P. Sci, 2024; 11 (12).
Number of Downloads : 10
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