Volume : 11, Issue : 12, December – 2024
Title:
DEVELOPMENT AND VALIDATION OF A REVERSE PHASE HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CARBIDOPA AND LEVODOPA IN PHARMACEUTICAL DOSAGE FORMS
Authors :
Venkatesh Bandra*, Dr.G.Vijay Kumar, B.Sravanthi
Abstract :
A simple, rapid, and efficient reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous determination of Carbidopa and Levodopa in pharmaceutical dosage forms. The analysis was performed using a Waters Alliance 2695 HPLC system equipped with a PDA Detector 996 model. The separation was achieved using a Phenomenex Gemini C18 column (4.6 × 250 mm, 5 µm), with an isocratic mobile phase consisting of acetonitrile and water (75:25%, v/v). The flow rate was set at 1 mL/min, with a column temperature of 40ºC, and detection was carried out at a wavelength of 240 nm. The injection volume was 10 µL, and the total run time was 6 minutes. The method was validated according to ICH guidelines for parameters such as specificity, linearity, precision, accuracy, and robustness. The method demonstrated good resolution and reliable quantification of both drugs, making it suitable for routine quality control analysis of Carbidopa and Levodopa in pharmaceutical formulations.
KEY WORDS: Carbidopa, Levodopa, HPLC, PDA Detector, quality control.
Cite This Article:
Please cite this article in press Venkatesh Bandra et al., Development And Validation Of A Reverse Phase Hplc Method For Simultaneous Determination Of Carbidopa And Levodopa In Pharmaceutical Dosage Forms.,Indo Am. J. P. Sci, 2024; 11 (12).
Number of Downloads : 10
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