Volume : 11, Issue : 11, November – 2024

Title:

QUALITATIVE AND QUANTITATIVE ANALYSIS OF LEAVES OF TINOSPORA CORDIFOLIA BY USING SOXHLATION AND MACERATION PROCESS AND THEIR ANTIOXIDANT ACTIVITY DETERMINATION

Authors :

Venkata Krishna Raju, Lakshmi Supriya Darapureddi, Aravind Eswar Iruvanti, Bhavya Sri Soma, Naga Poojitha Devarakonda

Abstract :

Tinospora cordifolia is refered as Giloe and Guduchii, with significant importance in the traditional medicinal systems. It is dioeciously plant, which is mostly used in Ayurvedic system and also known as a ‘Rasayans’ of medicinal system.it is develops immune system of the body and protect against infection. The aim of this study is carried out to analyse the phytochemical compounds in leaves and stem extracts of T. cordifolia by using phytochemical screening tests and estimate TFC by using aluminium chloride method in the sample extracts. The leaf extracts (maceration & soxhlation) of T. cordifolia expressed the presence of several phytochemicals viz., flavonoids, amino acids, diterpenes, protein, saponins and carbohydrates. The result of phytochemical screening tests revealed that diterpenes and carbohydrates are positive in all extracts of T. cordifolia, but flavonoids and saponins only present in methanol and ethanol extracts. TFC of T. cordifolia was higher in ethanolic leaves extracts than methanolic leaves extracts. The studies justify that T. cordifolia use in traditional medicines. The investigation further proposed that the phytochemicals present in leaves of T. cordifolia, which can be use as natural antioxidants in medicinal drugs. The antioxidant activity was also determined by using H2O2 scavenging.
Key Words: TFC – Total Flavonoid Content, NaOH – Sodium Hydroxide, AlCl3- Aluminium Chloride, Tinospora Cordifolia, V/v – volume per volume

Cite This Article:

Please cite this article in press Raju Venkata Krishna et al., Qualitative And Quantitative Analysis Of Leaves Of Tinospora Cordifolia By Using Soxhlation And Maceration Process And Their Antioxidant Activity Determination.,Indo Am. J. P. Sci, 2024; 11 (11).

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References:

1.Patwardhan B, Gautam M. Botanical immune drugs: scope and opportunities, Drug Discovery Today. 2005; 10(7):495– 502.
2. Sharma R, Amin H, Galib R, Prajapati PK. Therapeutic vistas of Guduchi (Tinospora cordifolia (Willd.) Miers): a medico historical memoir, Journal of Research and Education in Indian Medicine, 2014; XX(2):121-135
3. Sharma R, Amin H, Galib R, Prajapati PK. Antidiabetic appraisal of Guduchi (Tinospora cordifolia (Willd.) Miers): insightful exposition of ayurvedic claims, Rasamruta, 2014; 6(16):1-14
4. Anonymous. Wealth of India. A Dictionary of Indian Raw Materials and Industrial Products. 1st ed. Vol. 10. New Delhi: CSIR; 2003, p. 251-252.
5. Khosa RL, Prasad S. Pharmacognostical studies on Guduchi Tinospora cordifolia (Miers.), Journal of Research and Education in Indian Medicine, 1971; 6:261.
6.Joseph JA, Shukitt-Hale B, Denisova NA, Bielinski D, Martin A, McEwen JJ et al. Reversals of age-related declines in neuronal signal transduction, cognitive, and motor behavioral deficits with blueberry, spinach, or strawberry dietary supplementation, Journal of Neuroscience, 1999; 19:8114-21.
7.Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts, Journal of Applied Microbiology, 1999; 86(6):985
8.Mukherjee PK. Quality control of herbal drugs. 2nd Ed. Business Horizons; 2007.
9.Khandelwal KR. Practical pharmacognosy technique and experiments. 23nd Ed. Nirah Prakashan; 2005.
10.Kokate CK. Practical pharmacognosy, 4th Ed. Vallabh Prakashan; 2011.
11.Practical pharmacognosy khandelwal k.r.2005.
12.https://pharmagroww.com/soxhletextraction/#Laboy_Glass_Soxhlet_Extraction_Apparatus_Set_5550_with_soxhlet_Extraction_Tube_Allihn_Condenser_Extraction_Thimble_Apparatus_Set_Essential_Oil_Extractor_Organic_Chemistry_Lab_Glasswar.
13.Sushma Rudraswamy, Brinda Suhas Godhi, Detailed Understanding of Different Extraction Methods For the Research on Medicinal Plants, Indian Journal of Oral Health and Research,2021, XX:XX.
14.Mohammed Ali, Pharmacognosy and phytochemistry volume-1
15.Aiyelaagbe OO, Osamudiamen PM. Phytochemical screening of active compounds in Mangifera indica Leaves from Ibadan, Oyo State Plant Science Research, 2009; 2(1):11- 13.
16.Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants, Journal of Phytological Research, 2011; 3:10-14.
17.Dasgupta S, Parmar A, Patel H. Preliminary phytochemical studies of Kalanchoe gastonis-bonnieri, International Journal of Pharma and Bio Sciences, 2013; 4:550-7.
18.Jakopic J, Veberic R, Stampar F. Extraction of phenolic compounds from green walnuts fruits in different solvents, Acta Agriculturae Slovenica, 2009; 93(1):11-15.
19.Koffi E, Sea T, Dodehe Y, Soro S. Effect of solvent type on extraction of polyphenols from twenty three Ivorian plants, Journal of Animal Plant Sciences, 2010; 5 (3):550- 558.
20.Flavanoids ck kokate vallabh prakashan 2014. 45 Bapatla college of pharmacy Dept. of Pharmaceutical Chemistry
21.Olajuyigbe OO, Afolayan AJ. Flavonoid content and antioxidant property of the bark extract of ziziphus mucronata willd. Subsp. Mucronata willd, BMC Complementary and Alternative Medicine, 2011; 11:130.
22.Madhusweta Das, Sneha Sehwag, Antioxidant Activity: An Overview, Journal of Science & Technology,2013;2278-2249.
23.Kitts DD, Wijewickreme AN, Hu C. Antioxidant properties of a North American ginseng extract, Molecular and Cellular Biochemistry, 2000; 203(1-2):1-10.
24.Jang HD, Chang KS, Huang YS, Hsu CL, Lee SH, Su MS. Principal phenolic phytochemicals and antioxidant activities of three Chinese medicinal plants, Food Chemistry, 2007; 103:749-756.
25.Premanath R, Lakshmideri N. Studies on antioxidant activity of Tinospora cordifolia (Miers) leaves using invitro models, Journal of American Science, 2010; 6(10):736 743