Volume : 11, Issue : 11, November – 2024
Title:
A VALIDATED RP-HPLC METHOD FOR ESTIMATION OF TOFACITINIB IN BULK AND PHARMACEUTICAL DOSAGE FORM
Authors :
Kavali Shivakumar*, B. Raju
Abstract :
A validated simple, sensitive, specific and precise RP-HPLC method has been established for the determination of Tofacitinib in API and pharmaceutical dosage form. Chromatogram was run through Agilent C18 150 x 4.6 mm, 5m using mobile phase comprising of 0.1% Formic Acid: Acetonitrile in the ratio 50:50. The flow rate was maintained at 1.0ml/min, temperature at 30°C. Optimized wavelength selected was 268.0nm. Retention time of Tofacitinib was found to be 2.314 min. %RSD was found to be 0.9. It shows linear response in a concentration range of 2.5-15μg/ml with regression coefficient of 0.999, %Recovery was obtained as 100.10%, LOD and LOQ values obtained from regression equation were 0.15 and 0.44. The method precision for the determination of assay was 0.6%. So, the method is effective in regular Quality check of bulk and pharmaceutical formulations.
Key words: RP-HPLC, Tofacitinib Tablets, Validation, Liquid chromatography, Bulk
Cite This Article:
Please cite this article in press Kavali Shiva Kumar et al., A Validated Rp-Hplc Method For Estimation Of Tofacitinib In Bulk And Pharmaceutical Dosage Form.,Indo Am. J. P. Sci, 2024; 11 (11).
Number of Downloads : 10
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