Volume : 11, Issue : 10, October – 2024
Title:
RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF LAMIVUDINE & RALTEGRAVIR IN PURE & PHARMACEUTICAL DOSAGE FORM.
Authors :
N.Manila*, Mrs.T.Vijayalaxmi,K.Chaitanyaprasad,Dr.K.Shravankumar
Abstract :
simple, specific, precise, and efficient method for the Simultaneous estimation of Lamivudine and Raltegravir in pure and pharmaceutical dosage forms by a Reverse Phase-High Performance Liquid Chromatography method is developed and validated. Selected mobile phase were in a combination of ACN: Water (40:60% v/v). Optimized column is a Phenomenex Gemini C18 (4.6mm×250mm) 5µm particle size and at a flow rate of 1.0mL/min with detection wavelength at 255nm for Lamivudine and Raltegravir. In our study, the validation of analytical method for determination of Lamivudine and Raltegravir in pure and pharmaceutical dosage forms was performed in accordance the parameters including-system suitability, specificity, linearity of response, accuracy, precision (reproducibility & repeatability), robustness (change of wave length±2 nm). The method is validated according to ICH guidelines. The results obtained by RP-HPLC methods are rapid, accurate and precise. Therefore, proposed method can be used for routine analysis of Lamivudine and Raltegravir in the pure form as well as in combined pharmaceutical dosage form.
Keywords: Lamivudine and Raltegravir, HPLC, Method Development, Validation.
Cite This Article:
Please cite this article in press N.Manila et a.,l RP-HPLC Method Development And Validation For Estimation Of Lamivudine & Raltegravir In Pure & Pharmaceutical Dosage Form..,Indo Am. J. P. Sci, 2024; 11 (10).
Number of Downloads : 10
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