Volume : 11, Issue : 10, October – 2024
Title:
EVALUATION OF ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF OCIMUM SANCTUM
Authors :
Ms. Sarika H. Kandhare*, Mr. Mahesh D. Dhumal
Abstract :
Background: Ocimum sanctum (commonly known as Holy Basil or Tulsi) is a revered herb in traditional medicine, renowned for its diverse pharmacological properties. This study aimed to evaluate the anthelmintic activity of ethanolic extracts of Ocimum sanctum leaves against Pheretima posthuma (earthworms), which serves as a model organism for screening anthelmintic agents.
Methods: Fresh leaves of Ocimum sanctum were collected, authenticated, and processed to obtain an ethanolic extract using a Soxhlet apparatus. The anthelmintic activity was assessed by preparing extracts at concentrations of 20, 40, and 60 mg/ml. The time taken for paralysis and death of the earthworms was recorded and compared against a control (normal saline) and the standard drug, Albendazole (20 mg/ml).
Results: The ethanolic extract of Ocimum sanctum exhibited significant anthelmintic activity. The time for paralysis and death decreased with increasing extract concentration, with the highest efficacy observed at 60 mg/ml, where paralysis occurred within 28 minutes and death within 35 minutes. In comparison, the control group showed no activity, while Albendazole exhibited paralysis in 18 minutes and death in 28 minutes.
Conclusion: The findings suggest that the ethanolic extract of Ocimum sanctum leaves possesses promising anthelmintic properties, potentially validating its traditional use in herbal medicine. Further studies are warranted to isolate and characterize the active constituents responsible for this activity and to explore their mechanisms of action.
Keywords: Ocimum sanctum leaves, Albendazole, paralysis time, death time, anthelmintic activity, helminthiasis, traditional medicine.
Cite This Article:
Please cite this article in press Sarika H. Kandhare et al., Evaluation Of Anthelmintic Activity Of Ethanolic Extract Of Ocimum Sanctum ..,Indo Am. J. P. Sci, 2024; 11 (10).
Number of Downloads : 10
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