Volume : 11, Issue : 09, September – 2024
Title:
METHOD DEVELOPMENT FOR ESTIMATION OF MONTELUKAST SODIUM IN PLASMA BY LCMS/MS AND ITS VALIDATION
Authors :
A.Suresh *, B. Sathya prasad and J N Suresh Kumar
Abstract :
The purpose of this investigation was to develop a rapid, simple, sensitive, and selective LC-MS/MS method for the quantitative estimation of Montelukast sodium in less volume of human plasma using less volume of blood. It is also expected that this method would provide an efficient solution for pharmacokinetic, bioavailability, and/or bioequivalence studies of Montelukast sodium. The precision and accuracy for Montelukast at this concentration was found to be 3.10% and 97.24%. The method has been found to be reproducible by performing three Precision and Accuracy (P&A) batches consisting of one intra day batch and two inter day batches.A sensitive method that is precise and accurate over a linear assay range of of 5.032ng/mL to 60.2362ng/mL of the drug has been validated for the determination of Montelukast in Human plasma using LC-MS/MS Method. Use of stable labeled isotopes as internal standards helped us to obtain the consistent and reproducible results. Also, the method showed no matrix effect and limited variability in recovery between analyte and IS. The method utilized only 100 µL of plasma for sample processing. A simple SPE technique with direct injection (avoids drying, evaporation and reconstitution steps) for sample preparation, thereby significantly reduces the sample processing time. The total run time per analysis of each sample is 3.0 min which allows analysis of more samples in a single day.
Keywords: LC-MS/MS, Montelukast sodium, Precision , Accuracy
Cite This Article:
Please cite this article in press A. Suresh et al., Method development for estimation Of montelukast sodium in plasma by LCMS/MS and Its validation, Indo Am. J. P. Sci, 2024; 11 (09).
Number of Downloads : 10
References:
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Volume : 11, Issue : 09, September – 2024
Title:
THE EFFECT OF EQUIPPING AMBULANCES WITH RAPID COMMUNICATION MECHANISMS IN RESPONDING QUICKLY TO THE INJURED IN EMERGENCY SITUATIONS
Authors :
Zaher Ali Awadh Alshehri., MADINE MNAJI MOHAMMED ALSHAREIF . Yasser Yahya Amer Alhabis ., Mohammed Abdullah Awwadh Al-Harthi., Mohammed Mnaji Mohammed Alshareif., Othman Mabruk Faraj Alotaibi., Marzouq Masad Bin Hamdan Alhathla.
Abstract :
The current study aimed to identify the impact of equipping ambulances with rapid communication mechanisms on the response time for injured individuals in emergency situations. This was achieved by employing a descriptive analytical approach through the distribution of a questionnaire consisting of 500 emergency medical technicians to assess their opinions regarding the equipping of ambulances and rapid communication mechanisms. The study’s results indicated that there was no statistical significance attributed to professional and occupational experiences or the number of years of service concerning the responses of the sample on the questionnaire. The study presented several findings, including that technological advancements have facilitated the provision of higher quality communication means in ambulances, ensuring various solutions for rapid access to victims in emergencies. Furthermore, the implementation of precise location identification technology has enhanced response speed and helped avoid traffic congestion. The study’s results also indicated that the quicker the access to the accident scene, the greater the chances of survival for the injured, and that there is an effect of delayed response on rescue opportunities.
Key words: Technique, technology, response speed, communication methods, rapid access methods.
Cite This Article:
Please cite this article in press Zaher Ali Awadh Alshehri et al., The Effect Of Equipping Ambulances With Rapid Communication Mechanisms In Responding Quickly To The Injured In Emergency Situations, Indo Am. J. P. Sci, 2024; 11 (09).
Number of Downloads : 10
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