Volume : 11, Issue : 09, September – 2024
Title:
DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING HPLC ASSAY METHOD FOR FAMCICLOVIR IN MARKETED FORMULATIONS
Authors :
Dr. Chandrakant P. Suryawanshi , Dr. Rajendra D. Wagh
Abstract :
The study focused on developing and validating a stability-indicating HPLC method for the assay of Famciclovir in pharmaceutical formulations. The identity of Famciclovir was confirmed through UV-visible wavelength scanning and FT-IR spectroscopy, revealing characteristic peaks aligned with its chemical structure. The UV-Visible spectra displayed a maximum absorption at 315 nm, with a highly linear calibration curve (R² = 0.998) across varying concentrations. Method optimization included fine-tuning chromatographic conditions, leading to successful separation using an Eclipse Plus column and a mobile phase of acetonitrile, methanol, and glacial acetic acid (45:25:15 v/v/v) at a flow rate of 1 ml/min.
Validation of the method showed excellent linearity, accuracy, precision, and robustness, supported by system suitability tests that ensured appropriate chromatographic performance. The assay of Famciclovir in pharmaceutical formulations demonstrated consistent accuracy and precision over three consecutive days. Forced degradation studies, including acid/base hydrolysis, oxidation, and exposure to thermal and photolytic stress, confirmed the method’s stability-indicating properties. Despite significant degradation, Famciclovir was effectively separated from its degradation products, confirming the specificity of the developed method.
Keywords: Famciclovir, Marketed formulation, FT-IR, HPLC etc.
Cite This Article:
Please cite this article in press Chandrakant P. Suryawanshi et al., Development And Validation Of A Stability-Indicating HPLC Assay Method For Famciclovir In Marketed Formulations, Indo Am. J. P. Sci, 2024; 11 (09).
Number of Downloads : 10
References:
1. Candaele M., Candaele D., on behalf of the Famciclovir Herpes Zoster Clinical Study Group. Famciclovir: confirmed efficacy of 250 mg tid for the treatment of herpes zoster infection. Seventh International Conference on Antiviral Research (Abstract 118), Charlesston, South Carolina, 27 Feb 4 March 1994.
2. Tyring S., Engst R., Corriveau C., Robillard N., Trottire S., Van Slycken S., Crann R. A., Locke L. A., Saltzman R., Palestine A. G., Br. J. Ophthalmol., 85, 576-581 (2001).
3. Perry C. M., Wagstaff A. J., Drugs, 50, 396-415 (1995).
4. Vere Hodge R. A., Cheng Y.-C., Antiviral Chem. Chemother., 4 (Suppl. 1), 13-24 (1993).
5. Arabian F. A., Sacks S. L., Drugs, 52, 17-32 (1996).
6. Pue M. A., Benet L. Z., Antiviral Chem. Chemother., 4 (Suppl. 1), 47—55 (1993).
7. Murray A. B., Antiviral Chem. Chemother., 6, 34-38 (1995).
8. Vere Hodge R. A., Sutton D., Boyd M. R., Harnden M. R., Jarvest R. L., Antimicrob. Agents Chemother., 33, 1765-1773 (1989).
9. Schenkel F., Rudaz S., Daali Y., Kondo Oestreicher M., Veuthey L., Dayer P., J. Chromatogr. B, 826, 1-7 (2005).
10. Brand B., Reese C. B., Song Q., Visintin C., Tetrahedron, 55, 5239— 5252 (1999).
11. Zhang Y. S., Wang L. Q., Guo Y. J., Cui J. G., Li J., Zhongguo Yiyao Gongye Zazhi, 33, 454-457 (2002).
12. Validation of analytical procedures, Proceedings of the International Conference on Harmonization (ICH). Commission of the Japan (1997).
13. A. Loregian, R. Gaatti, G. Palu and Elico F. De Palo, Biomed. Sci. Application, 764, 289-311 (2001).
14. S. Nizamuddian, D. Goli, Y. N. Manohara and M. C. Ravi, Asian J. Chem., 19(5), 3617-3620 (2007).
15. K. V. Subramanyam, P. Mohanraj, V. S. Saravanan and N. Gopal, Asian J. Chem., 19(6), 4911-4913 (2007).
16. D. G. Sankar, N. Sujatha, B. A. Kumar and P. V. M. Latha, Asian J. Chem., 19(2), 1602-1604 (2007).
17. J. Zhang, West China J. Pharmaceut. Sci., 21, 302-303 (2006).
18. S. Nizamuddin, B. M. Gurupadayya, M. C. Ravi, Y. N. Manohara and S. Appala Raju, Indian J. Pharmaceut. Sci., 69(3), 451-453 (2007).
19. Jin O. Hung and Z. Y., Chinese J. Biochem. Pharmaceut., 22(4), 1891-1896 (2001).
20. H. Zongyu and J. Ou, Chinese J. Biochem. Pharmaceut., 20(2), 111-113 (2000).
21. V. Srinivas, M. Narasimha Rao, A. Appa Rao and G. Srinubabu, E-J. Chem., 5(1), 58-67 (2008).
22. W. Shui Wang and G. Zu Qin, Chinese J. Antibiotics, 32(3), 159-162 (2007).
23. ICH, Q2A, Text on Validation of Analytical Procedures, International Conference on Harmonization. Geneva, 15 (1994), ICH, Q2B, Text on Validation of Analytical Procedures: Methodology, International Conference on Harmonization, Geneva (1996) pp. 1-8.
24. International Federation of Pharmaceutical Manufactures & Associations (IFPMA), Validation of Analytical Procedures: Text and Methodology, in Proceedings of the International Conference on Harmonization (ICH ’96), Methodology Q2(R1), Geneva, Switzerland (1996).
25. United States Pharmacopoeia (USP), XXVI. Validation of Compendial Methods. United States Pharmacopoeial Convention Inc., Rockville, MD, USA (2003).
Volume : 11, Issue : 09, September – 2024
Title:
THE EFFECT OF EQUIPPING AMBULANCES WITH RAPID COMMUNICATION MECHANISMS IN RESPONDING QUICKLY TO THE INJURED IN EMERGENCY SITUATIONS
Authors :
Zaher Ali Awadh Alshehri., MADINE MNAJI MOHAMMED ALSHAREIF . Yasser Yahya Amer Alhabis ., Mohammed Abdullah Awwadh Al-Harthi., Mohammed Mnaji Mohammed Alshareif., Othman Mabruk Faraj Alotaibi., Marzouq Masad Bin Hamdan Alhathla.
Abstract :
The current study aimed to identify the impact of equipping ambulances with rapid communication mechanisms on the response time for injured individuals in emergency situations. This was achieved by employing a descriptive analytical approach through the distribution of a questionnaire consisting of 500 emergency medical technicians to assess their opinions regarding the equipping of ambulances and rapid communication mechanisms. The study’s results indicated that there was no statistical significance attributed to professional and occupational experiences or the number of years of service concerning the responses of the sample on the questionnaire. The study presented several findings, including that technological advancements have facilitated the provision of higher quality communication means in ambulances, ensuring various solutions for rapid access to victims in emergencies. Furthermore, the implementation of precise location identification technology has enhanced response speed and helped avoid traffic congestion. The study’s results also indicated that the quicker the access to the accident scene, the greater the chances of survival for the injured, and that there is an effect of delayed response on rescue opportunities.
Key words: Technique, technology, response speed, communication methods, rapid access methods.
Cite This Article:
Please cite this article in press Zaher Ali Awadh Alshehri et al., The Effect Of Equipping Ambulances With Rapid Communication Mechanisms In Responding Quickly To The Injured In Emergency Situations, Indo Am. J. P. Sci, 2024; 11 (09).
Number of Downloads : 10
References:
1- Gulsen, M. F., Kurt, M., Kaleli, I., & Ulasti, A. (2020). Personal protective equipment (ppe) using in antalya 112 emergency ambulance services during outbreak. medRxiv, 2020-06.
2- Lyng, J., Adelgais, K., Alter, R., Beal, J., Chung, B., Gross, T., … & Yee, A. (2021). Recommended essential equipment for basic life support and advanced life support ground ambulances 2020: a joint position statement. Pediatrics, 147(6).
3- Rosiello, F., Zelinotti, L., Monti, M., Cosmi, P., Procacciante, M., Petrelli, G., … & Serale, M. (2020). Decontamination of ambulance and equipment, a problem for worker safety and national security. In 2nd Scientific International Conference on CBRNe SICC Series| 2020 Epidemics, biological threats, and radiological events. The importance of a multidisciplinary approach for International Research Cooperation Book of Abstract (pp. 69-69). tab editore.
4- Tiwary, B., Nilima, N., Majumdar, P., Singh, M., & Khan, M. A. (2020). Quality of services provided by public funded ambulance program: experience from a northern state in India. Clinical epidemiology and global health, 8(3), 962-966.
5- Chen, L., Tang, T. Q., Song, Z., Guo, R. Y., & Huang, H. J. (2022). Empirical investigation of child evacuation under non-emergency and emergency situations. Journal of Transportation Safety & Security, 14(4), 585-606.
6- Xakimovich, A. S., & Qaxramonjon o’g’li, Z. H. (2022). Prediction of Situations That May Occur in Emergency Situations of Bridges by Means of Optical Sensors. Texas Journal of Engineering and Technology, 13, 55-59.
7- Kyrkou, C., Kolios, P., Theocharides, T., & Polycarpou, M. (2022). Machine learning for emergency management: A survey and future outlook. Proceedings of the IEEE, 111(1), 19-41.
8- Idland, S., Iversen, E., Brattebø, G., Kramer-Johansen, J., & Hjortdahl, M. (2022). From hearing to seeing: medical dispatchers’ experience with use of video streaming in medical emergency calls− a qualitative study. BMJ open, 12(12), e063395.
9- Alarilla, A., Stafford, M., Coughlan, E., Keith, J., & Tallack, C. (2022). Why have ambulance waiting times been getting worse?. Health Found.
10- Olani, A., Beza Demisse, L., Alemayehu, M., & Sultan, M. (2022). Factors Associated with Willingness to Call Emergency Medical Services for Worsening Symptoms of COVID-19 in Addis Ababa, Ethiopia. Open Access Emergency Medicine, 563-572.
11- Rabeea’h, W. A., Snooks, H., Porter, A., Khanom, A., Cole, R., Edwards, A., … & Watkins, A. (2022). STRategies to manage Emergency ambulance Telephone Callers with sustained High needs: an Evaluation using linked Data (STRETCHED)–a study protocol. BMJ open, 12(3), e053123.
12- Marincowitz, C., Stone, T., Hasan, M., Campbell, R., Bath, P. A., Turner, J., … & Goodacre, S. (2022). Accuracy of emergency medical service telephone triage of need for an ambulance response in suspected COVID-19: an observational cohort study. BMJ open, 12(5), e058628.
13- Brady, L., Gilson, L., George, A., Vries, S. D., & Hartley, S. (2024). A community-based ambulance model: lessons for emergency medical services and everyday health systems resilience from South Africa. Health policy and planning, czae070.
14- Wennlund, K. T., Kurland, L., Olanders, K., Khoshegir, A., Al Kamil, H., Castrén, M., & Bohm, K. (2022). Emergency medical dispatchers’ experiences of managing emergency calls: a qualitative interview study. BMJ open, 12(4), e059803.
15- GÖKÇEDAĞ, Ş. Y. (2023). AMBULANCE ACCIDENTS BETWEEN 2016-2021 AND THEIR INVESTIGATION IN TERMS OF OCCUPATIONAL HEALTH AND SAFETY1. Current Research in Health Sciences.
16- Mulyono, N. B., Pambudi, N. F., Ahmad, L. B., & Adhiutama, A. (2022). Determining response time factors of emergency medical services during the COVID-19 pandemic. International Journal of Emergency Services, 11(1), 154-167.