Volume : 12, Issue : 08, August- 2025

Title:

ECOFRIENDLY SPECTROSCOPIC METHOD DEVELOPMENT FOR SIMULTANEOUS ESTIMATION OF ANTIHYPERTENSIVE DRUGS IN COMBINED DOSAGE FORM

Authors :

Yadav Rishikesh Rajbahadur*, Deepak Kumar Basedia, B. K. Dubey, Prabhat Kumar Jain, Sunil Shah

Abstract :

The present study focuses on the development of an ecofriendly spectroscopic method for the simultaneous estimation of antihypertensive drugs in a combined dosage form. The method was designed to be simple, cost-effective, and environmentally sustainable by avoiding the use of hazardous organic solvents and minimizing chemical waste. Using UV-visible spectroscopy, optimized wavelengths were selected for accurate quantification of the drugs without interference from excipients. The proposed method was validated as per International Conference on Harmonisation (ICH) guidelines for parameters such as linearity, accuracy, precision, specificity, limit of detection (LOD), and limit of quantitation (LOQ). Recovery studies at three concentration levels (80%, 100%, and 120%) confirmed the method’s accuracy, with percentage recovery values within the acceptable range for both drugs. The ecofriendly nature of the method not only ensures environmental safety but also offers a sustainable alternative for routine quality control analysis in the pharmaceutical industry.
Keywords: Ecofriendly method, UV-visible spectroscopy, simultaneous estimation, antihypertensive drugs, green analytical chemistry, method validation, recovery studies, sustainable analysis.

Cite This Article:

Please cite this article in press Yadav Rishikesh Rajbahadur et al., Ecofriendly Spectroscopic Method Development For Simultaneous Estimation Of Antihypertensive Drugs In Combined Dosage Form., Indo Am. J. P. Sci, 2025; 12(08).

Number of Downloads : 10

References:

1. Chobanian AV, et al. Seventh report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. Hypertension. 2003;42(6):1206–1252.
2. Whelton PK, et al. 2017 ACC/AHA/AAPA/ABC/ACPM/AGS/APhA/ASH/ASPC/NMA/PCNA Guideline for the prevention, detection, evaluation, and management of high blood pressure in adults. J Am Coll Cardiol. 2018;71(19):e127–e248.
3. Adegoke OA, et al. A new second derivative spectrophotometric method for the determination of amlodipine besylate in tablets. J Taibah Univ Sci. 2014;8(4):343–351.
4. Dinc E, et al. Simultaneous spectrophotometric determination of losartan potassium and hydrochlorothiazide in tablets by chemometric methods. J Pharm Biomed Anal. 2002;28(3-4):591–600.
5. Gałuszka A, et al. The 12 principles of green analytical chemistry and the SIGNIFICANCE mnemonic of green analytical practices. TrAC Trends Anal Chem. 2013;50:78–84.
6. Płotka-Wasylka J. A new tool for the evaluation of the analytical procedure: Green Analytical Procedure Index. Talanta. 2018;181:204–209.
7. Smith EL, et al. Deep eutectic solvents (DESs) and their applications. Chem Rev. 2014;114(21):11060–11082.
8. Florindo C, et al. Deep eutectic solvents: Overcoming 21st-century challenges. Curr Opin Green Sustain Chem. 2019;18:31–36.
9. ICH. Validation of analytical procedures: Text and methodology Q2(R1). International Conference on Harmonisation; 2005.
10. Płotka-Wasylka J, et al. Complex GAPI, A green analytical procedure index for complex analytical procedures. Green Chem. 2021;23(3):865–873.
11. International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH). ICH Harmonised Tripartite Guideline Q2(R1): Validation of Analytical Procedures: Text and Methodology. Geneva: ICH; 2005.